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BioVector? LS 8 細(xì)胞系是研究**牙釉質(zhì)形成（成釉細(xì)胞生物學(xué)）**最常用的經(jīng)典模型。細(xì)胞系數(shù)據(jù)表 (Cell Line Datasheet) - 中文版 1. 基本信息細(xì)胞名稱: LS 8 (有時寫作 LS8) 物種: 小鼠 (Mus musculus) 細(xì)胞類型: 成釉細(xì)胞 (Ameloblast-like cells) 起源背景: 建立自小鼠切牙的成釉器上皮組織，通過 SV40 大 T 抗原永生化而成。生物安全等級: 1 (由于使用了 SV40 永生化，建議在 BSL-2 實(shí)驗(yàn)室操作以確保安全) 2. 核心生物學(xué)特征表型特征: 具有前成釉細(xì)胞 (Pre-ameloblast) 的特征，能夠表達(dá)牙釉質(zhì)基質(zhì)蛋白，如 Amelogenin (釉原蛋白)。分子特征: 表達(dá)成釉細(xì)胞特異性標(biāo)志物，包括釉原蛋白、釉蛋白 (Enamelin) 和成釉蛋白 (Ameloblastin)。主要應(yīng)用: 牙齒發(fā)育研究: 研究牙釉質(zhì)基質(zhì)的分泌及礦化機(jī)制。毒理學(xué): 評估氟化物（如氟中毒研究）或雙酚 A (BPA) 對成釉細(xì)胞功能的毒性影響。信號通路: 研究 Wnt、BMP 和 TGF-β 等通路在牙齒上皮分化中的作用。 3. 培養(yǎng)參數(shù) 形態(tài)學(xué): 類上皮狀，呈多角形，貼壁生長。培養(yǎng)基: DMEM + 10% 胎牛血清 (FBS) + 1% 青鏈霉素 (P/S)。培養(yǎng)條件: 37 °C, 5% CO?。傳代建議: 細(xì)胞達(dá)到 80-90% 融合時使用 Trypsin-EDTA 消化，傳代比例通常為 1:3 至 1:5。冷凍保存: 90% FBS + 10% DMSO。 Cell Line Datasheet - English Version 1. General Information Cell Line Name: LS 8 (also written as LS8) Species: Mouse (Mus musculus) Cell Type: Ameloblast-like cells Origin: Established from the enamel organ epithelium of mouse incisors, immortalised using the SV40 Large T-antigen. Biosafety Level: 1 (BSL-2 handling is recommended due to SV40 immortalisation). 2. Key Biological Features Phenotype: Exhibits characteristics of pre-ameloblasts; capable of synthesizing enamel matrix proteins. Molecular Profile: Expresses key ameloblast markers, including Amelogenin, Enamelin, and Ameloblastin. Research Applications: Dental Development: Studying the mechanisms of enamel matrix secretion and biomineralization. Toxicology: Modeling the effects of environmental factors, such as fluoride (dental fluorosis) or endocrine disruptors like BPA, on enamel formation. Signalling Pathways: Investigating the roles of Wnt, BMP, and TGF-β pathways in odontogenic epithelial differentiation. 3. Culture Specifications Morphology: Epithelial-like; polygonal cells growing as an adherent monolayer. Culture Medium: DMEM supplemented with 10% FBS and 1% Pen-Strep. Incubation: 37 °C with 5% CO?. Subculture: Use Trypsin-EDTA for dissociation when cells reach 80–90% confluency; recommended split ratio of 1:3 to 1:5. Storage/Freezing: 90% FBS + 10% DMSO. 技術(shù)提醒 (Technical Tip): LS 8 細(xì)胞對血清質(zhì)量較為敏感，可能會影響其釉原蛋白的表達(dá)水平。在進(jìn)行礦化誘導(dǎo)實(shí)驗(yàn)時，通常需要額外添加 β-甘油磷酸鈉和抗壞血酸。

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