BioVector? PIG3V 人白癜風(fēng)黑素細(xì)胞永生化細(xì)胞系 / Human Vitiligo Melanocyte Immortalized Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? PIG3V 細(xì)胞
中文名稱: BioVector? PIG3V 人白癜風(fēng)黑素細(xì)胞永生化細(xì)胞系
英文名稱: BioVector? PIG3V Human Vitiligo Melanocyte Immortalized Cell Line
產(chǎn)品類別: 皮膚疾病研究細(xì)胞系
物種來源: 人 (Homo sapiens)
組織來源: 皮膚；表皮
細(xì)胞類型: 黑素細(xì)胞 (Melanocyte)
疾病: 白癜風(fēng) (Vitiligo)
患者信息: 白癜風(fēng)供體 Ma9308P4
構(gòu)建方法: 采用攜帶HPV16 E6和E7基因的逆轉(zhuǎn)錄病毒載體LXSN16E6E7轉(zhuǎn)染白癜風(fēng)患者來源的原代黑素細(xì)胞，經(jīng)遺傳霉素篩選和克隆純化后建立
形態(tài)特性: 樹突狀黑素細(xì)胞形態(tài)
生長(zhǎng)特性: 貼壁生長(zhǎng)
特性特征:

1. 永生化特性: 已傳代超過100次群體倍增，遠(yuǎn)超正常成人黑素細(xì)胞（最多50次倍增）

2. 白癜風(fēng)特征保留: 超微結(jié)構(gòu)顯示擴(kuò)大的內(nèi)質(zhì)網(wǎng)，這是白癜風(fēng)黑素細(xì)胞的特征性結(jié)構(gòu)

3. 蛋白質(zhì)滯留: 擴(kuò)大的內(nèi)質(zhì)網(wǎng)中存在分子量為37.5、47.5和56.5 kDa的蛋白質(zhì)滯留

4. 黑色素生成: 酪氨酸酶加工正常，黑素體保持色素沉著，表型上更接近正常黑素細(xì)胞而非黑色素瘤細(xì)胞

5. miR-211表達(dá)缺失: PIG3V細(xì)胞中miR-211表達(dá)嚴(yán)重下調(diào)，而其靶基因PPARGC1A、RRM2和TAOK1表達(dá)上調(diào)

6. 線粒體功能缺陷: 線粒體復(fù)合物I、II和IV功能缺陷，呼吸反應(yīng)異常，氧消耗率降低

7. 氧化應(yīng)激: 活性氧產(chǎn)生增加，對(duì)氧化應(yīng)激敏感

8. Nrf2信號(hào): Nrf2信號(hào)通路受損，過表達(dá)Nrf2可促進(jìn)線粒體生物合成和功能

9. 研究應(yīng)用: 廣泛用于白癜風(fēng)發(fā)病機(jī)制研究、氧化應(yīng)激研究、miRNA功能研究、線粒體功能障礙研究、自身抗原轉(zhuǎn)運(yùn)研究、藥物篩選等
   培養(yǎng)基: BioVector? PIG3V 細(xì)胞專用完全培養(yǎng)基（推薦基礎(chǔ)成分：Medium 254基礎(chǔ)培養(yǎng)基，添加1% HMGS-2黑素細(xì)胞生長(zhǎng)補(bǔ)充劑、10%胎牛血清和1%青霉素-鏈霉素）
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 當(dāng)細(xì)胞融合度達(dá)到70-80%時(shí)，推薦使用 BioVector? 專用傳代試劑（如0.25%胰蛋白酶-EDTA）進(jìn)行消化，按1:2至1:4比例進(jìn)行傳代，每2-3天換液一次
   凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90%胎牛血清 + 10% DMSO）
   質(zhì)控載體: BioVector? pUC19 質(zhì)粒
   宿主菌: BioVector? DH5α 化學(xué)感受態(tài)細(xì)胞
   質(zhì)量控制: 支原體檢測(cè)：陰性；無菌檢測(cè)：陰性；HPV16 E6/E7基因檢測(cè)：陽(yáng)性；白癜風(fēng)特征驗(yàn)證：內(nèi)質(zhì)網(wǎng)擴(kuò)張
   儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存
   生物安全等級(jí): 1（需在二級(jí)生物安全柜內(nèi)操作）
   僅供科研使用

English:
BioVector? PIG3V Human Vitiligo Melanocyte Immortalized Cell Line Datasheet

Product Name: BioVector? PIG3V Cells
Chinese Name: BioVector? PIG3V 人白癜風(fēng)黑素細(xì)胞永生化細(xì)胞系
English Name: BioVector? PIG3V Human Vitiligo Melanocyte Immortalized Cell Line
Product Category: Skin Disease Research Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Skin; Epidermis
Cell Type: Melanocyte
Disease: Vitiligo
Patient Information: Vitiligo donor Ma9308P4
Derivation: Established by transfecting primary melanocytes derived from a vitiligo patient with the retroviral construct LXSN16E6E7 carrying HPV16 E6 and E7 genes, followed by geneticin selection and clonal purification
Morphology: Dendritic melanocyte morphology
Growth Characteristics: Adherent
Characteristics:

1. Immortalization Properties: Has undergone more than 100 population doublings since establishment, significantly exceeding the maximum of 50 doublings for normal adult melanocytes

2. Vitiligo Feature Retention: Ultrastructural characterization reveals dilated endoplasmic reticulum profiles, characteristic of vitiligo melanocytes

3. Protein Retention: Retention of proteins with molecular weights of 37.5, 47.5, and 56.5 kDa within the dilated endoplasmic reticulum

4. Melanogenesis: Tyrosinase is processed properly, melanosomes remain pigmented, and the phenotype resembles melanocytes rather than melanoma cells in culture

5. miR-211 Deficiency: PIG3V cells show severely downregulated miR-211 expression, with reciprocal upregulation of its target genes PPARGC1A, RRM2, and TAOK1

6. Mitochondrial Dysfunction: Defects in mitochondrial complexes I, II, and IV; abnormal respiratory responses; reduced oxygen consumption rate

7. Oxidative Stress: Increased reactive oxygen species production; sensitive to oxidative stress

8. Nrf2 Signaling: Impaired Nrf2 signaling pathway; overexpression of Nrf2 promotes mitochondrial biosynthesis and function

9. Research Applications: Widely used in vitiligo pathogenesis research, oxidative stress studies, miRNA function studies, mitochondrial dysfunction research, autoantigen transport studies, and drug screening
   Culture Medium: BioVector? Complete Medium for PIG3V Cells (Recommended composition: Medium 254 basal medium supplemented with 1% HMGS-2 melanocyte growth supplement, 10% fetal bovine serum, and 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: When cells reach 70-80% confluence, recommended to use BioVector? Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:2 to 1:4. Medium renewal is recommended every 2-3 days
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Control Vector: BioVector? pUC19 Plasmid
   Host Strain: BioVector? DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; HPV16 E6/E7 Gene Detection: Positive; Vitiligo Feature Validation: Dilated endoplasmic reticulum
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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