BioVector? CIHP-1 人腎臟足細(xì)胞系 / Human Kidney Podocyte Cell Line 數(shù)據(jù)說(shuō)明書(shū)

產(chǎn)品名稱: BioVector? CIHP-1 細(xì)胞
同義詞: BioVector? CIHP1
中文名稱: BioVector? CIHP-1 人腎臟足細(xì)胞系
英文名稱: BioVector? CIHP-1 Human Kidney Podocyte Cell Line
產(chǎn)品類別: 腎細(xì)胞系；條件永生化細(xì)胞系
物種來(lái)源: 人 (Homo sapiens)
組織來(lái)源: 腎臟；腎小球
細(xì)胞類型: 足細(xì)胞 (Podocyte)
構(gòu)建方法: 通過(guò)溫度敏感性SV40LT抗原轉(zhuǎn)基因構(gòu)建的條件永生化細(xì)胞系
永生化機(jī)制: 在許可溫度（33°C）下，SV40LT抗原表達(dá)，細(xì)胞增殖；在非許可溫度（37°C）下，SV40LT抗原失活，細(xì)胞停止分裂并啟動(dòng)分化程序，獲得成熟足細(xì)胞表型
形態(tài)特性: 分化條件下呈現(xiàn)典型的足細(xì)胞形態(tài)，具有足突樣結(jié)構(gòu)
生長(zhǎng)特性: 貼壁生長(zhǎng)；溫度依賴性生長(zhǎng)
特征標(biāo)志物: 表達(dá)成熟足細(xì)胞特異性標(biāo)志蛋白，包括nephrin、podocin、CD2AP、synaptopodin
特性特征:

1. 分化潛能: 在37°C分化條件下，細(xì)胞獲得與體內(nèi)成熟足細(xì)胞高度相似的生理表型

2. 均質(zhì)性: 提供均質(zhì)、穩(wěn)定、可長(zhǎng)期培養(yǎng)的細(xì)胞來(lái)源

3. 研究應(yīng)用: 廣泛用于腎臟生理學(xué)、毒性學(xué)研究、代謝研究、藥物靶向篩選、共培養(yǎng)模型（如3D培養(yǎng)）、糖尿病腎病、急性腎損傷、足細(xì)胞凋亡機(jī)制等研究領(lǐng)域

4. 文獻(xiàn)支持: 已被全球超過(guò)100篇同行評(píng)審出版物引用，是國(guó)際公認(rèn)的足細(xì)胞研究模型
   培養(yǎng)基: BioVector? CIHP-1 細(xì)胞專用完全培養(yǎng)基（推薦基礎(chǔ)成分：RPMI-1640培養(yǎng)基，添加10%胎牛血清、1%胰島素-轉(zhuǎn)鐵蛋白-硒、1%青霉素-鏈霉素）
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；增殖培養(yǎng)溫度：33°C；分化培養(yǎng)溫度：37°C
   傳代方法: 當(dāng)細(xì)胞融合度達(dá)到80-90%時(shí)，推薦使用 BioVector? 專用傳代試劑（如0.25%胰蛋白酶-EDTA）進(jìn)行消化，按1:3至1:6比例進(jìn)行傳代

凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90%胎牛血清 + 10% DMSO）
質(zhì)量控制: 支原體檢測(cè)：陰性；無(wú)菌檢測(cè)：陰性；標(biāo)志物表達(dá)驗(yàn)證：陽(yáng)性
儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存
生物安全等級(jí): 1（需在二級(jí)生物安全柜內(nèi)操作）
僅供科研使用

English:
BioVector? CIHP-1 Human Kidney Podocyte Cell Line Datasheet

Product Name: BioVector? CIHP-1 Cells
Synonyms: BioVector? CIHP1
Chinese Name: BioVector? CIHP-1 人腎臟足細(xì)胞系
English Name: BioVector? CIHP-1 Human Kidney Podocyte Cell Line
Product Category: Kidney Cell Line; Conditionally Immortalized Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Kidney; Glomerulus
Cell Type: Podocyte
Derivation: Conditionally immortalized cell line generated using a temperature-sensitive SV40LT antigen transgene
Immortalization Mechanism: At the permissive temperature (33°C), the SV40LT antigen is expressed, allowing cell proliferation. At the non-permissive temperature (37°C), the SV40LT antigen is inactivated, cells cease division, and initiate a differentiation program, acquiring a mature podocyte phenotype
Morphology: Exhibits typical podocyte morphology upon differentiation, with foot process-like structures
Growth Characteristics: Adherent; Temperature-dependent growth
Marker Expression: Expresses mature podocyte-specific marker proteins, including nephrin, podocin, CD2AP, and synaptopodin
Characteristics:

1. Differentiation Potential: Under differentiating conditions at 37°C, cells acquire a physiological phenotype highly analogous to mature podocytes in vivo

2. Homogeneity: Provides a homogeneous, stable, and long-lasting cell source

3. Research Applications: Widely used in renal physiology, toxicology studies, metabolism research, drug targeting screening, co-culture models (e.g., 3D culture), diabetic nephropathy, acute kidney injury, and podocyte apoptosis mechanism studies

4. Literature Support: Cited in over 100 peer-reviewed publications worldwide; an internationally recognized model for podocyte research
   Culture Medium: BioVector? Complete Medium for CIHP-1 Cells (Recommended composition: RPMI-1640 medium supplemented with 10% fetal bovine serum, 1% Insulin-Transferrin-Selenium, 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Proliferation Culture Temperature: 33°C; Differentiation Culture Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector? Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:3 to 1:6
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Marker Expression Validation: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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