RPTEC/TERT1 Cell Line細(xì)胞系

Product Name / 產(chǎn)品名稱:
BioVector? RPTEC/TERT1 Cell Line
BioVector? RPTEC/TERT1細(xì)胞系

Description / 描述:
The BioVector? RPTEC/TERT1 (human Renal Proximal Tubule Epithelial/Telomerase Reverse Transcriptase 1) cell line is a well-characterized, telomerase-immortalized human cell line derived from the proximal tubule of a healthy adult male kidney -4-10. It was established by transfecting primary renal proximal tubule epithelial cells with the human telomerase reverse transcriptase (hTERT) gene, which allows the cells to bypass replicative senescence while retaining the key morphological and functional characteristics of their primary cell counterparts -10. This results in a stable, reproducible, and physiologically relevant in vitro model of the human renal proximal tubule -5-7.
BioVector? RPTEC/TERT1（人腎近端腎小管上皮/端粒酶逆轉(zhuǎn)錄酶1） 細(xì)胞系是一種特性明確、經(jīng)端粒酶永生化的人源細(xì)胞系，來源于健康成年男性供體的腎近端小管 -4-10。它通過將人端粒酶逆轉(zhuǎn)錄酶基因轉(zhuǎn)入原代腎近端小管上皮細(xì)胞而建立，使細(xì)胞能夠繞過復(fù)制性衰老，同時保留其原代細(xì)胞的關(guān)鍵形態(tài)和功能特征 -10。這使其成為一個穩(wěn)定、可重復(fù)且生理相關(guān)性高的人腎近端小管體外模型 -5-7。

Species / 物種:
Human / 人

Tissue Origin / 組織來源:
Kidney; Proximal Tubule (Cortex) / 腎臟；近端腎小管（皮質(zhì)）

Morphology / 形態(tài):
Epithelial-like / 上皮樣

Growth Characteristics / 生長特性:
Adherent; forms characteristic "domes" at high density, indicating functional cell polarization and vectorial transport -4-10.
貼壁生長；高密度時形成特征性的"穹窿"結(jié)構(gòu)，表明細(xì)胞功能極化和定向轉(zhuǎn)運能力 -4-10。

Key Applications / 主要應(yīng)用:

• Nephrotoxicity screening (drugs, environmental toxicants like cadmium) -3-5-7

• Drug transporter studies -1-6

• 3D cell culture and kidney-on-a-chip models -4-7

• High-throughput screening -4-6

• Biogerontology and tissue engineering -10

• Studying renal progenitor cell biology and tubular regeneration -3

• 腎毒性篩選（藥物、環(huán)境毒物如鎘）-3-5-7

• 藥物轉(zhuǎn)運蛋白研究 -1-6

• 3D細(xì)胞培養(yǎng)及腎臟芯片模型 -4-7

• 高通量篩選 -4-6

• 生物老年學(xué)及組織工程 -10

• 研究腎祖細(xì)胞生物學(xué)及腎小管再生 -3

Key Features & Functional Characteristics / 關(guān)鍵特征與功能特性:

• Primary-like Function / 類原代功能: Retains many differentiated features of primary proximal tubule cells, including response to parathyroid hormone (PTH) but not to arginine vasopressin (AVP), sodium-dependent phosphate uptake, and functional megalin/cubilin-mediated endocytosis system -4-10.

• Barrier Integrity / 屏障完整性: Forms tight junctions and maintains a stable Trans-Epithelial Electrical Resistance (TEER) when cultured on permeable supports, demonstrating intact functional barrier -4-7.

• Transporter Expression / 轉(zhuǎn)運蛋白表達(dá): Expresses key proximal tubule transporters and markers, such as Aminopeptidase N (CD13), γ-Glutamyl Transferase (GGT), and E-cadherin -4. Transporter expression has been shown to mature over time in culture -7.

• Progenitor Population / 祖細(xì)胞亞群: Contains a subpopulation of cells co-expressing CD133 and CD24, which exhibit progenitor/stem cell-like properties, such as forming nephrospheres and differentiating into multiple lineages -3.

• 保留了原代近端小管細(xì)胞的許多分化特征，包括對甲狀旁腺激素有反應(yīng)但對精氨酸加壓素?zé)o反應(yīng)、鈉依賴性磷酸鹽攝取以及功能性巨蛋白/立方蛋白介導(dǎo)的內(nèi)吞系統(tǒng) -4-10。

• 在通透膜上培養(yǎng)時能形成緊密連接并維持穩(wěn)定的跨上皮電阻，顯示出完整的功能性屏障 -4-7。

• 表達(dá)關(guān)鍵的近端小管轉(zhuǎn)運蛋白和標(biāo)志物，如氨基肽酶N、γ-谷氨酰轉(zhuǎn)移酶和E-鈣黏蛋白 -4。研究表明，轉(zhuǎn)運蛋白的表達(dá)會隨著培養(yǎng)時間的延長而成熟 -7。

• 包含一個共表達(dá)CD133和CD24的細(xì)胞亞群，該亞群表現(xiàn)出祖細(xì)胞/干細(xì)胞的特性，例如形成腎球和分化為多種譜系的能力 -3。

Engineered Variants / 工程改造細(xì)胞株:

Culture Conditions / 培養(yǎng)條件:

Quality Control / 質(zhì)量控制:
Negative for mycoplasma and bacterial contamination. Verified for morphology, expression of epithelial markers (e.g., pan-cytokeratin, E-cadherin), and functional characteristics (e.g., dome formation) -4-6.
無支原體及細(xì)菌污染。經(jīng)驗證具有正常形態(tài)，表達(dá)上皮標(biāo)志物（如泛角蛋白、E-鈣黏蛋白），并具有功能特性（如形成穹窿）-4-6。

Storage / 存儲:

• BioVector? Cryopreservation Medium (with 10% DMSO) is recommended for long-term storage in liquid nitrogen vapor phase -4.

• 推薦使用 BioVector? 細(xì)胞凍存液（含10% DMSO）進(jìn)行長期保存（液氮氣相）-4。

BioVector? RPTEC Growth Kit
BioVector? RPTEC生長因子添加套裝

Contains the necessary supplements (EGF, hydrocortisone, insulin, transferrin, etc.) to be added to BioVector? Renal Epithelial Cell Growth Medium for complete RPTEC/TERT1 culture.
包含必要的添加物，需添加至 BioVector? 腎上皮細(xì)胞生長培養(yǎng)基 中，以完成RPTEC/TERT1的培養(yǎng)。

BioVector? G418 Sulfate Solution
BioVector? G418硫酸鹽溶液

For maintaining selective pressure on the parental RPTEC/TERT1 cell line.
用于維持親本RPTEC/TERT1細(xì)胞系的選擇性壓力。

BioVector? Trypsin-EDTA for Primary Cells
BioVector? 原代細(xì)胞胰蛋白酶-EDTA消化液

Gentle enzyme solution for dissociating adherent RPTEC/TERT1 monolayers.
用于解離貼壁RPTEC/TERT1單層細(xì)胞的溫和酶溶液。

BioVector? Soybean Trypsin Inhibitor
BioVector? 大豆胰蛋白酶抑制劑

Used to neutralize trypsin activity during subculture to protect cell viability.
用于傳代過程中中和胰蛋白酶活性，保護(hù)細(xì)胞活力。

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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