ciPTEC Cell Line ciPTEC細(xì)胞系

Product Name / 產(chǎn)品名稱:
BioVector? ciPTEC Cell Line
BioVector? ciPTEC細(xì)胞系

Description / 描述:
The BioVector? ciPTEC (conditionally immortalized proximal tubule epithelial cell) line is a well-characterized human cell model derived from urine or kidney tissue. It is conditionally immortalized using a temperature-sensitive SV40 large T antigen and human telomerase (hTERT), allowing for proliferation at 33°C and differentiation/maturation at 37°C. These cells retain key morphological and functional characteristics of the renal proximal tubule, including the expression of tight junctions, specific transporters, and the ability to perform endocytosis.
BioVector? ciPTEC（條件性永生化近端腎小管上皮細(xì)胞） 是一種來源于尿液或腎組織的人源細(xì)胞系，是研究腎臟生理和藥物轉(zhuǎn)運(yùn)的經(jīng)典模型。通過溫度敏感性SV40大T抗原和端粒酶實(shí)現(xiàn)條件性永生化，使其能在33°C增殖，在37°C分化成熟。該細(xì)胞保留了近端腎小管的關(guān)鍵形態(tài)和功能特征，包括表達(dá)緊密連接、特異性轉(zhuǎn)運(yùn)蛋白以及具有內(nèi)吞能力。

Species / 物種:
Human / 人

Tissue Origin / 組織來源:
Proximal Tubule of the Nephron (Kidney) / 腎單位近端小管（腎臟）

Morphology / 形態(tài):
Epithelial / 上皮細(xì)胞樣

Growth Characteristics / 生長特性:
Conditionally immortalized (Adherent) / 條件性永生化（貼壁生長）

• Proliferation Phase: 33°C

• Maturation Phase: 37°C

• 增殖階段：33°C

• 成熟階段：37°C

Key Applications / 主要應(yīng)用:

• Nephrotoxicity screening / 腎毒性篩選

• Drug transport studies (OCT2, P-gp, MRP4, BCRP) / 藥物轉(zhuǎn)運(yùn)研究

• Physiological reabsorption studies / 生理性重吸收研究

• Disease modeling (e.g., kidney aging, senescence) / 疾病模型（如腎臟衰老）

• 3D culture and kidney-on-a-chip / 3D培養(yǎng)及腎臟芯片

Key Transporters Expressed / 表達(dá)的主要轉(zhuǎn)運(yùn)蛋白:

• Endogenous: OCT2, P-gp, MRP4, BCRP, OAT4

• Engineered Variants Available: OAT1, OAT3

• 內(nèi)源性表達(dá)：OCT2, P-gp, MRP4, BCRP, OAT4

• 工程改造過表達(dá)株系：OAT1, OAT3

Culture Conditions / 培養(yǎng)條件:

Quality Control / 質(zhì)量控制:
Negative for mycoplasma and bacterial contamination. Validated for proper morphology and expression of proximal tubule markers (AQP1, ZO-1).
無支原體及細(xì)菌污染。經(jīng)驗(yàn)證具有正常形態(tài)并表達(dá)近端腎小管標(biāo)志物（AQP1, ZO-1）。

Storage / 存儲(chǔ):

• BioVector? Cryopreservation Medium is recommended for long-term storage in liquid nitrogen vapor phase.

• 推薦使用 BioVector? 細(xì)胞凍存液 進(jìn)行長期保存。

BioVector? ciPTEC-OAT1 Cell Line
BioVector? ciPTEC-OAT1過表達(dá)細(xì)胞系

A variant engineered to stably express the Organic Anion Transporter 1 (OAT1), specifically designed for studying anionic drug transport and nephrotoxicity.
穩(wěn)定表達(dá)有機(jī)陰離子轉(zhuǎn)運(yùn)蛋白1（OAT1）的改造株，專為研究陰離子藥物轉(zhuǎn)運(yùn)和腎毒性設(shè)計(jì)。

BioVector? ciPTEC-OAT3 Cell Line
BioVector? ciPTEC-OAT3過表達(dá)細(xì)胞系

A variant engineered to stably express the Organic Anion Transporter 3 (OAT3), ideal for anionic compound interaction studies.
穩(wěn)定表達(dá)有機(jī)陰離子轉(zhuǎn)運(yùn)蛋白3（OAT3）的改造株，用于陰離子化合物相互作用研究。

BioVector? Renal Epithelial Cell Growth Medium Kit
BioVector? 腎上皮細(xì)胞生長培養(yǎng)基套裝

Contains basal medium and necessary supplements for optimal growth of ciPTEC and other renal epithelial cells.
包含基礎(chǔ)培養(yǎng)基及必需添加物，用于ciPTEC及其他腎上皮細(xì)胞的最佳生長。

BioVector? Fetal Bovine Serum
BioVector? 胎牛血清

Qualified for supporting the conditional proliferation of ciPTEC cell lines.
經(jīng)驗(yàn)證適用于支持ciPTEC細(xì)胞系的條件性增殖。

BioVector? Trypsin-EDTA Solution
BioVector? 胰蛋白酶-EDTA消化液

For gentle and efficient detachment of adherent ciPTEC monolayers.
用于溫和高效地消化貼壁的ciPTEC單層細(xì)胞。

BioVector? Cryopreservation Medium
BioVector? 細(xì)胞凍存液

Serum-free, defined formulation for high-viability cryopreservation of ciPTEC.
無血清、成分明確配方，用于ciPTEC細(xì)胞的高活力凍存。

Supplier生產(chǎn)商：

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

電話:400-800-2947

工作QQ/微信同號:1843439339

E-mail: [email protected]

網(wǎng)址 http://www.nedfriskphoto.com