BioVector? N/TERT-1 人永生化角質(zhì)形成細(xì)胞系 / BioVector? N/TERT-1 Human Immortalized Keratinocyte Cell Line

通用定義 / General Definition:BioVector? N/TERT-1 是一種廣泛使用的人源永生化皮膚角質(zhì)形成細(xì)胞系。該細(xì)胞系通過在原代人包皮角質(zhì)形成細(xì)胞中穩(wěn)定表達(dá)端粒酶逆轉(zhuǎn)錄酶 (hTERT) 并結(jié)合 p16INK4a 表達(dá)缺失而建立。與傳統(tǒng)的通過病毒癌基因（如 HPV-16 E6/E7）轉(zhuǎn)化的細(xì)胞系不同，N/TERT-1 在很大程度上保留了原代角質(zhì)形成細(xì)胞的生物學(xué)特性，包括正常的核型、對分化信號的反應(yīng)能力以及在器官型培養(yǎng)中形成分層表皮的能力。它被認(rèn)為是研究皮膚生物學(xué)、毒理學(xué)和皮膚疾病體外建模的理想選擇。

BioVector? N/TERT-1 is a widely used immortalized human skin keratinocyte cell line. It was established by stably expressing the human telomerase reverse transcriptase (hTERT) in primary human foreskin keratinocytes, coupled with the spontaneous loss of p16INK4a expression. Unlike traditional lines transformed by viral oncogenes (such as HPV-16 E6/E7), N/TERT-1 largely retains the biological characteristics of primary keratinocytes, including a normal karyotype, responsiveness to differentiation signals, and the ability to form a stratified epidermis in organotypic cultures. It is considered an ideal model for studying skin biology, toxicology, and in vitro modeling of dermatological diseases.

BioVector? N/TERT-1 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? N/TERT-1 人永生化角質(zhì)形成細(xì)胞

• 組織來源： 人新生兒包皮組織

• 永生化機(jī)制： hTERT 穩(wěn)定表達(dá) + p16 缺失

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 典型的鋪路石狀上皮形態(tài)

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? K-SFM（角質(zhì)形成細(xì)胞無血清培養(yǎng)基），含牛垂體提取物 (BPE) 和表皮生長因子 (EGF)。

• 備選培養(yǎng)基：DFK 培養(yǎng)基（DMEM/F-12 1:1 混合，添加特定生長因子）。

• 鈣濃度控制： 維持低鈣濃度（通常小于 0.1 mM）以保持細(xì)胞處于未分化的增殖狀態(tài)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2。

• 傳代比例： 1:3 至 1:6；當(dāng)匯合度達(dá)到 70%-80% 時必須傳代，以防止自發(fā)分化。

3. 細(xì)胞應(yīng)用

• 皮膚替代模型： 用于構(gòu)建 3D 有機(jī)型皮膚等效模型 (Organotypic Skin Equivalents, OSE)，研究表皮屏障功能。

• 毒理學(xué)測試： 評估化妝品、藥物或化學(xué)物質(zhì)對皮膚的刺激性和毒性。

• 炎癥研究： 通過刺激（如 TNF-alpha）研究角質(zhì)形成細(xì)胞的細(xì)胞因子分泌和信號通路。

4. 注意事項(xiàng)

• 密度敏感性： N/TERT-1 對細(xì)胞密度非常敏感。匯合度過高會觸發(fā)鈣依賴性分化過程，導(dǎo)致細(xì)胞停止增殖并脫落。

• 消化過程： 使用 0.05% Trypsin-EDTA 消化，一旦細(xì)胞變圓變亮即加入胰酶抑制劑或含血清培養(yǎng)基終止。

English Datasheet

1. General Product Information

• Product Name: BioVector? N/TERT-1 Human Immortalized Keratinocyte Cell Line

• Source: Human neonatal foreskin tissue

• Immortalization: hTERT stable expression + p16INK4a loss

• Growth Properties: Adherent

• Morphology: Classic cobblestone epithelial morphology

2. Culture Conditions

• Basal Medium: BioVector? K-SFM (Keratinocyte Serum-Free Medium) supplemented with Bovine Pituitary Extract (BPE) and Human Recombinant EGF.

• Alternative Medium:DFK medium (DMEM/F-12 mix with specific growth factors).

• Calcium Regulation: Maintain low calcium levels (typically < 0.1 mM) to keep cells in a proliferative, undifferentiated basal state.

• Incubation: 37°C, 5% CO2.

• Subculturing: 1:3 to 1:6 ratio; subculture at 70%-80% confluence to avoid density-induced differentiation.

3. Applications

• 3D Skin Modeling: Widely used in air-liquid interface (ALI) cultures to generate stratified human skin equivalents.

• Dermatotoxicity: Assessing the safety and irritancy of topical formulations or environmental pollutants.

• Gene Editing: An excellent platform for CRISPR/Cas9 or RNAi studies due to its consistent growth and primary-like genetic profile.

4. Key Usage Notes

• Differentiation Control: Because these cells retain the ability to differentiate, maintaining them in a sub-confluent state is critical for long-term culture stability.

• Coating: While they generally attach well to tissue-culture treated plastic, coating with Collagen I or IV can improve attachment and expansion in certain experimental setups.

注意 / Note: N/TERT-1 應(yīng)在生物安全二級 (BSL-2) 條件下操作。該細(xì)胞系是研究銀屑病、特應(yīng)性皮炎等皮膚病病理機(jī)制的極佳體外工具。N/TERT-1 should be handled under Biosafety Level 2 (BSL-2) conditions. This cell line is an excellent in vitro tool for investigating the pathological mechanisms of skin diseases such as psoriasis and atopic dermatitis.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

電話:400-800-2947

工作QQ/微信同號:1843439339

網(wǎng)址

http://www.nedfriskphoto.com