BioVector? mHypoE-N1 小鼠下丘腦神經元細胞系 / mHypoE-N1 Mouse Hypothalamic Neuronal Cell Line

通用定義 / General Definition:BioVector? mHypoE-N1 是一種源自成年小鼠下丘腦的永生化神經元細胞系。該細胞系通過逆轉錄病毒介導的 SV40 T-antigen 轉化技術建立。mHypoE-N1 保持了下丘腦神經元的許多關鍵生理特征，包括對各種激素（如胰島素、瘦素）和神經遞質的反應能力。它是研究神經內分泌調控、能量代謝平衡、晝夜節(jié)律以及下丘腦信號轉導機制的理想體外模型。

BioVector? mHypoE-N1 is an immortalized neuronal cell line derived from the adult mouse hypothalamus. This cell line was established using retroviral-mediated SV40 T-antigen transformation. mHypoE-N1 cells retain many key physiological characteristics of hypothalamic neurons, including responsiveness to various hormones (such as insulin and leptin) and neurotransmitters. It serves as an ideal in vitro model for studying neuroendocrine regulation, energy homeostasis, circadian rhythms, and hypothalamic signaling mechanisms.

BioVector? mHypoE-N1 技術說明書 (Technical Datasheet)

中文版說明書

1. 產品基本信息

• 產品名稱： BioVector? mHypoE-N1 小鼠下丘腦神經元細胞

• 細胞來源： 小鼠 (Mouse / Mus musculus)

• 組織來源： 下丘腦 (Hypothalamus)

• 生長特性： 貼壁生長

• 細胞形態(tài)： 神經元樣（具有胞體和軸突樣突起）

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? 高糖 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

• 傳代比例： 1:3 至 1:5；建議在細胞達到 80% 匯合時進行傳代，避免細胞過密導致分化狀態(tài)改變。

3. 細胞應用

• 代謝調節(jié)研究： 研究瘦素 (Leptin) 和胰島素 (Insulin) 信號通路對神經元的影響。

• 神經內分泌學： 探索下丘腦激素的合成、分泌及其調節(jié)機制。

• 篩選實驗： 評估穿透血腦屏障的藥物對中樞神經系統(tǒng)的潛在作用。

4. 注意事項

• 細胞狀態(tài)： 神經元細胞系對培養(yǎng)環(huán)境較為敏感，請確保使用預熱的培養(yǎng)基并輕柔操作。

• 鑒定驗證： 建議定期檢測神經元標志物（如截短型神經絲蛋白或特定的下丘腦神經肽基因表達）以確認細胞表型。

English Datasheet

1. General Product Information

• Product Name: BioVector? mHypoE-N1 Mouse Hypothalamic Neuronal Cell Line

• Organism: Mouse (Mus musculus)

• Tissue Source: Hypothalamus

• Growth Properties: Adherent

• Morphology: Neuronal (exhibiting cell bodies and neurite-like processes)

2. Culture Conditions

• Basal Medium: BioVector? High-glucose DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:5 ratio; it is recommended to subculture when cells reach 80% confluence to prevent changes in differentiation state due to overcrowding.

3. Applications

• Metabolic Regulation: Investigating the impact of Leptin and Insulin signaling pathways on neuronal function.

• Neuroendocrinology: Exploring the mechanisms governing the synthesis, secretion, and regulation of hypothalamic hormones.

• Screening Assays: Evaluating the central nervous system effects of drugs designed to cross the blood-brain barrier.

4. Key Usage Notes

• Culture Sensitivity: Neuronal cell lines are sensitive to environmental shifts. Always use pre-warmed media and handle the cells gently during dissociation.

• Phenotypic Validation: Periodically monitoring neuronal markers or the expression of specific hypothalamic neuropeptide genes is recommended to ensure the functional integrity of the BioVector? mHypoE-N1 line.

Note: For experiments requiring high transfection efficiency, BioVector? recommends using specialized neuronal transfection reagents. To maintain the long-term stability of the cell line, ensure the use of BioVector? validated FBS and minimize the number of passages.

Supplier生產廠家: BioVector NTCC典型培養(yǎng)物保藏中心

E-mail: [email protected]

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