BioVector? BL-89 人鼻咽癌細胞系 / BL-89 Human Nasopharyngeal Carcinoma Cell Line

通用定義 / General Definition:BioVector? BL-89 是一種源自人類鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的上皮細胞系。鼻咽癌在組織學(xué)上通常分為角化性和非角化性，而 BL-89 常被用作研究鼻咽癌惡性演變、腫瘤免疫微環(huán)境以及病毒相關(guān)發(fā)病機制（如 EB 病毒的影響）的體外模型。該細胞系具有較強的增殖能力和成瘤性，是頭頸部腫瘤學(xué)研究中不可或缺的工具。

BioVector? BL-89 is an epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). NPC is histologically categorized into keratinizing and non-keratinizing types; BL-89 is frequently utilized as an in vitro model to study malignant progression, the tumor immune microenvironment, and virus-related pathogenesis (such as the influence of the Epstein-Barr Virus). Characterized by its robust proliferative capacity and tumorigenicity, this cell line is an essential tool in head and neck oncology research.

BioVector? BL-89 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? BL-89 人鼻咽癌細胞

• 組織來源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生長特性： 貼壁生長

• 細胞形態(tài)： 上皮樣 (Epithelial-like)，呈多角形，貼壁緊密

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，恒溫加濕

• 傳代比例： 1:3 至 1:5；建議在細胞匯合度達到 80%-90% 時進行傳代。

3. 細胞應(yīng)用

• 致癌機制研究： 探索調(diào)控 NPC 細胞周期、凋亡及自噬的分子通路。

• 藥物耐受性分析： 建立針對順鉑、 5-氟尿嘧啶等常用化療藥的耐藥模型。

• 侵襲與轉(zhuǎn)移： 研究上皮-間充質(zhì)轉(zhuǎn)化 (EMT) 在鼻咽癌擴散中的作用。

4. 注意事項

• 身份鑒定： 建議實驗室定期進行 STR 鑒定，以區(qū)分 BL-89 與其他相似的 NPC 細胞系（如 CNE 系列）。

• 細胞凍存： 建議使用 BioVector? 專用無血清細胞凍存液，以確保復(fù)蘇后的高活力。

English Datasheet

1. General Product Information

• Product Name: BioVector? BL-89 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like, polygonal, tightly adherent

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Humidified Atmosphere

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Oncogenesis Mechanisms: Exploring molecular pathways regulating the cell cycle, apoptosis, and autophagy in NPC cells.

• Drug Resistance Analysis: Establishing models for resistance against common chemotherapeutics like Cisplatin or 5-Fluorouracil.

• Invasion and Metastasis: Investigating the role of Epithelial-Mesenchymal Transition (EMT) in the dissemination of nasopharyngeal cancer.

4. Key Usage Notes

• Authentication: Labs are encouraged to perform regular STR profiling to distinguish BL-89 from other similar NPC lines (e.g., the CNE series).

• Cryopreservation: BioVector? recommends using specialized serum-free freezing media to ensure maximum viability upon recovery.

Note: BL-89 cells should be handled under Biosafety Level 2 (BSL-2) protocols. For consistent results in signaling studies, BioVector? recommends using validated FBS and avoiding over-confluence during routine maintenance.

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

電話:400-800-2947

工作QQ/微信同號:1843439339

網(wǎng)址

http://www.nedfriskphoto.com