BioVector? BL-60 人鼻咽癌細(xì)胞系 / BL-60 Human Nasopharyngeal Carcinoma Cell Line

通用定義 / General Definition:BioVector? BL-60 是一種源自人類鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的上皮細(xì)胞系。與 BL-89 類似，它是研究鼻咽惡性腫瘤的重要實(shí)驗(yàn)工具。該細(xì)胞系在體外表現(xiàn)出穩(wěn)定的生長特性，被廣泛應(yīng)用于基礎(chǔ)腫瘤生物學(xué)研究，包括細(xì)胞增殖動(dòng)力學(xué)、腫瘤相關(guān)抗原的表達(dá)、以及對各種抗癌活性成分（如化療藥物、合成小分子或植物提取物）的細(xì)胞毒性評價(jià)。

BioVector? BL-60 is an epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). Similar to BL-89, it serves as an important experimental tool for studying nasopharyngeal malignancies. This cell line exhibits stable growth characteristics in vitro and is widely used in basic tumor biology research, including cell proliferation kinetics, the expression of tumor-associated antigens, and cytotoxicity evaluations of various anti-cancer active ingredients (such as chemotherapeutics, synthetic small molecules, or plant extracts).

BioVector? BL-60 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? BL-60 人鼻咽癌細(xì)胞

• 組織來源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮樣 (Epithelial-like)

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，恒溫加濕

• 傳代比例： 1:3 至 1:5；建議在細(xì)胞匯合度達(dá)到 85% 左右時(shí)傳代。

3. 細(xì)胞應(yīng)用

• 抗癌藥物篩選： 建立體外模型篩選具有潛在抗 NPC 活性的候選藥物。

• 生物標(biāo)志物探索： 研究 NPC 細(xì)胞表面的特異性受體或胞內(nèi)信號分子。

• 細(xì)胞死亡機(jī)制： 觀察藥物誘導(dǎo)的凋亡、壞死或自噬過程。

4. 注意事項(xiàng)

• 支原體預(yù)防： 建議每兩周進(jìn)行一次支原體檢測，確保培養(yǎng)體系的潔凈。

• 細(xì)胞異質(zhì)性： 在長期傳代過程中，細(xì)胞表型可能發(fā)生微小漂移，建議在實(shí)驗(yàn)中使用低代數(shù)細(xì)胞。

English Datasheet

1. General Product Information

• Product Name: BioVector? BL-60 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Humidified Atmosphere

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach approximately 85% confluence.

3. Applications

• Anti-cancer Drug Screening: Establishing in vitro models to screen drug candidates with potential anti-NPC activity.

• Biomarker Exploration: Investigating specific cell surface receptors or intracellular signaling molecules in NPC cells.

• Cell Death Mechanisms: Observing drug-induced apoptosis, necrosis, or autophagy processes.

4. Key Usage Notes

• Mycoplasma Prevention: Periodic mycoplasma testing (e.g., every two weeks) is recommended to ensure the purity of the culture system.

• Phenotypic Stability: To minimize genetic and phenotypic drift associated with long-term culture, BioVector? recommends using low-passage cells for definitive experiments.

Note: BL-60 cells should be handled under Biosafety Level 2 (BSL-2) protocols. For consistent experimental outcomes, BioVector? suggests the use of its validated high-purity FBS and standardized RPMI-1640 medium.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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