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產(chǎn)品分類
| NEB（New England Biolabs） |
| pMXB10 |
| 原核細胞表達載體 |
| -- |
| T7 |
| 多克隆位點，限制性內(nèi)切酶 |
| 7816bp |
| -- |
| -- |
| -- |
| 氨芐青霉素(Ampicillin) |
| -- |
| -- |
| E6903 |
| -- |
| -- |
| -- |
載體質(zhì)粒圖譜和多克隆位點信息
載體簡介
E. coli malE gene, encoding the maltose binding protein (MBP, 42 kDa) (3), fused in-frame to
the coding region of the Mxe gyrase A intein-chitin binding domain (28 kDa) from pTXB1 (2,4,5).
pMXB10 can be used to test plasmid transformation, cell culture, induction and purification
procedures. After induction with 0.3 mM IPTG at 30°C for 3 hours (or 15°C for 12-16 hours),
100 ml of cells should yield 2-3 mg of a 70 kDa fusion protein. After chitin column purification
and cleavage, approximately 1.5-2.0 mg of MBP is usually obtained. This double stranded vector
is 7813 bp in length.
載體序列
DEFINITION Control vector pMXB10, complete sequence.
ACCESSION
VERSION
KEYWORDS .
SOURCE Control vector pMXB10
ORGANISM Control vector pMXB10
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7816)
AUTHORS Telenti,A., Southworth,M., Alcaide,F., Daugelat,S., Jacobs,W.R. Jr.
and Perler,F.B.
TITLE The Mycobacterium xenopi GyrA protein splicing element:
characterization of a minimal intein
JOURNAL J Bacteriol 179, 6378-6382 (1997)
PUBMED 9335286
REFERENCE 2 (bases 1 to 7816)
AUTHORS Evans,T.C. Jr., Benner,J. and Xu,M.-Q.
TITLE Semisynthesis of cytotoxic proteins using a modified protein
splicing element
JOURNAL Protein Sci 7, 2256-2264 (1998)
PUBMED 9827992
REFERENCE 3 (bases 1 to 7816)
AUTHORS Southworth,M.W., Amaya,K., Evans,T.C., Xu,M.-Q. and Perler,F.B.
TITLE Purification of proteins fused to either the amino or carboxy
terminus of the Mycobacterium xenopi Gyrase A intein
JOURNAL BioTechniques 27, 110-120 (1999)
PUBMED 10407673
REFERENCE 4 (bases 1 to 7816)
AUTHORS Perler,F.B.
TITLE InBase, the intein database
JOURNAL Nucleic Acids Res 30 (1), 383-384 (2002)
PUBMED 11752343
REFERENCE 5 (bases 1 to 7816)
AUTHORS Ghosh,I. and Xu,M.Q.
TITLE Direct Submission
JOURNAL Submitted (23-OCT-2007) Research Department, New England Biolabs,
240 County Road, Ipswich, MA 01938, USA
FEATURES Location/Qualifiers
source 1..7816
/organism="Control vector pMXB10"
/mol_type="other DNA"
gene 140..1000
/gene="bla"
CDS 140..1000
/gene="bla"
/note="ampR (confers resistance to ampicillin)"
/codon_start=1
/product="beta-lactamase"
sig_peptide 140..208
/gene="bla"
/note="required for secretion to the periplasm; cleaved
off to form the mature beta-lactamase protein"
rep_origin 1042..1555
/note="M13 origin of replication (+ -)"
rep_origin 1666..2254
/note="pMB1 origin of replication (clockwise) (RNAII -35
to RNA/DNA switch point)"
gene complement(2623..2814)
/gene="rop"
CDS complement(2623..2814)
/gene="rop"
/exception="alternative start codon"
/codon_start=1
/transl_table=11
/product="ROP protein"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
ADELYRSCLARFGDDGENL"
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