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產(chǎn)品分類
pCDFDuet1載體基本信息
| EMD Biosciences (Novagen) |
| pCDFDuet1, pCDFDuet 1 |
| 大腸桿菌蛋白雙表達載體，有兩個多克隆位點 |
| 高 |
| 多克隆位點，限制性內(nèi)切酶 |
| 3781 bp |
| T7 |
| T7: 5'-TAATACGACTCACTATAGGG-3'; |
| T7t |
| T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
| N-His, C-S |
| streptomycin |
pCDFDuet-1 is designed for the coexpression of two target genes. pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA). |
| 71340-3 |
| 瞬時表達 Transient |
| 組成型 Constitutive |
| 非病毒 |
pCDFDuet1載體質(zhì)粒圖譜和多克隆位點信息
pCDFDuet1載體簡介
pCDFDue-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.
pCDFDuet1載體序列
DEFINITION pCDFDuet
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3781
/organism="pCDFDuet"
/mol_type="other DNA"
misc_feature 2..29
/label="lacO"
promoter 214..232
/label="T7_promoter"
misc_feature 232..259
/label="lacO"
misc_feature complement(448..466)
/label="T7_Terminal_primer"
terminator 467..502
/label="T7_terminator"
gene complement(680..1471)
/label="spect (variant)"
/gene="spect (variant)"
CDS complement(680..1471)
/label="ORF frame 1"
CDS complement(680..1471)
/label="ORF frame 1"
promoter complement(1513..1541)
&
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