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pGEXKG載體基本信息
| 出品公司: | ATCC |
|---|---|
| 別名: | pGEX-KG, pGEXKG, pGEX KG |
| 質(zhì)粒類型: | 大腸桿菌蛋白表達(dá)載體 |
| 表達(dá)水平: | 高拷貝 |
| 啟動子: | Tac |
| 克隆方法: | 多克隆位點，限制性內(nèi)切酶, BamHI，SmaI, EcoRI, XbaI, NcoI, SalI, XhoI, SacI, HindIII |
| 載體大小: | 5006 bp |
| 5' 測序引物及序列: | pGEX5': GGGCTGGCAAGCCACGTTTGGTG |
| 3' 測序引物及序列: | pGEX3': CCGGGAGCTGCATGTGTCAGAGG |
| 載體標(biāo)簽: | N-GST |
| 載體抗性: | Ampicillin 氨芐 |
| 備注: | 復(fù)制子是pMB1, 來源于pGEX-2T |
| 產(chǎn)品目錄號: | 77103 |
| 穩(wěn)定性: | 瞬時表達(dá) Transient |
| 組成型: | 誘導(dǎo)表達(dá) |
| 病毒/非病毒: | 非病毒 |
pGEXKG載體質(zhì)粒圖譜和多克隆位點信息
pGEXKG載體簡介
pGEXKG載體序列
LOCUS pGEX-KG 5006 bp DNA circular SYN 23-SEP-2012
DEFINITION pGEX-KG.
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.nedfriskphoto.com/
COMMENT VNTAUTHORNAME|biovector.net|FEATURES Location/Qualifiers
source 1..5006
/organism="pGEX-KG"
/mol_type="other DNA"
/vntifkey="98"
promoter 184..212
/vntifkey="30"
/label=tac_promoter
misc_feature 224..246
/vntifkey="21"
/label=M13_pUC_rev_primer
CDS 258..1010
/vntifkey="4"
/label=ORF\frame\3
misc_feature 258..1014
/vntifkey="21"
/label=GST
misc_feature 869..891
/vntifkey="21"
/label=pGEX_5_primer
misc_feature complement(1056..1078)
/vntifkey="21"
/label=pGEX_3_primer
promoter 1344..1372
/vntifkey="30"
/label=AmpR_promoter
CDS 1414..2274
/gene="Ampicillin"
/vntifkey="4"
/label=Ampicillin
/note="ORF frame 1"
gene 1414..2274
/gene="Ampicillin"
/vntifkey="60"
rep_origin 2429..3048
/vntifkey="33"
/label=pBR322_origin
misc_feature 3346..4437
/vntifkey="21"
/label=lacI
CDS 3478..4437
/vntifkey="4"
/label=ORF\frame\1
promoter 4486..4515
/vntifkey="30"
/label=lac_promoter
misc_feature 4529..4551
/vntifkey="21"
/label=M13_pUC_rev_primer
misc_feature 4550..4568
/vntifkey="21"
/label=M13_reverse_primer
misc_feature complement(4580..4596)
/vntifkey="21"
/label=M13_forward20_primer
misc_feature complement(4589..4611)
/vntifkey="21"
/label=M13_pUC_fwd_primer
misc_feature 4577..4732
/vntifkey="21"
/label=lacZ_a
BASE COUNT 1234 a 1205 c 1302 g 1265 t
ORIGIN
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gttccgcgtg gatccccggg aatttccggt ggtggtggtg
961 gaattctaga ctccatgggt cgactcgagc tcaagcttaa ttcatcgtga ctgactgacg
1021 atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
1081 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
1141 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
1201 tataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
1261 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
1321 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
1381 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
1441 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
1501 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
1561 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
1621 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
1681 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
1741 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
1801 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
1861 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
1921 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
1981 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
2041 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
2101 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
2161 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
2221 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
2281 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
2341 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
2401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
2461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
2521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
2581 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
2641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
2701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
2761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
2821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
2881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
2941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
3001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
3061 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
3121 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
3181 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
3241 acgcatctgt gcggtatttc acaccgcata aattccgaca ccatcgaatg gtgcaaaacc
3301 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
3361 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
3421 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
3481 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
3541 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
3601 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
3661 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
3721 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
3781 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
3841 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
3901 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
3961 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
4021 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
4081 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
4141 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
4201 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
4261 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
4321 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
4381 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
4441 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
4501 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
4561 tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
4621 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
4681 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
4741 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
4801 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
4861 acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
4921 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
4981 gaattatttt tgatggcgtt ggaatt
//
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產(chǎn)品分類
pGEXKG載體基本信息
| ATCC |
| pGEX-KG, pGEXKG, pGEX KG |
| 大腸桿菌蛋白表達(dá)載體 |
| 高拷貝 |
| Tac |
| 多克隆位點，限制性內(nèi)切酶, BamHI，SmaI, EcoRI, XbaI, NcoI, SalI, XhoI, SacI, HindIII |
| 5006 bp |
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