pSI質(zhì)粒載體圖譜序列抗性說(shuō)明書(shū)價(jià)格報(bào)價(jià)
- 價(jià) 格：￥0
- 貨 號(hào)：40322795496
- 產(chǎn) 地：北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作微信:1843439339 (QQ同號(hào))
手機(jī)：18901268599
地址：北京
- 已注冊(cè)
質(zhì)粒載體菌株細(xì)胞株基因保藏中心-Biovector Science Lab,Inc.可提供數(shù)萬(wàn)種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純化、病毒包裝、基因沉默RNAi等服務(wù)。作為美國(guó)質(zhì)粒載體菌株細(xì)胞株基因保藏中心分庫(kù)及唯一辦事處，Biovector Inc.可為您提供便捷一站式的產(chǎn)品進(jìn)口服務(wù)，到貨快捷，步驟簡(jiǎn)便。通過(guò)Google搜索"Biovector+您所需資源名稱(chēng)",您一定能找到所需的資源和技術(shù)服務(wù)。如有需要請(qǐng)聯(lián)系：
Dr Xu,Senior Scientist
電郵[email protected]
電話18901268599
訂購(gòu)合同下載請(qǐng)點(diǎn)擊：Biovector質(zhì)粒載體菌株細(xì)胞基因訂購(gòu)合同-Biovector Science Lab
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Biovector,Inc質(zhì)粒載體菌株細(xì)胞株基因保藏中心列表網(wǎng)址http://www.nedfriskphoto.com
標(biāo)簽：以下資源由Biovector Inc. 質(zhì)粒載體菌株細(xì)胞株基因保藏中心獨(dú)家供應(yīng)并發(fā)布，侵權(quán)必究。Biovector,Inc質(zhì)粒載體菌株細(xì)胞株基因保藏中心列表網(wǎng)址http://biovector.blog.163.com 質(zhì)粒 載體 索取 報(bào)價(jià) 價(jià)格 優(yōu)惠 售價(jià) 出售 圖譜 序列 酶切位點(diǎn) 轉(zhuǎn)化 構(gòu)建 求購(gòu) 購(gòu)買(mǎi) 訂購(gòu) sequence map plasmid vector gene strain order ship 抗性 resistance restriction site產(chǎn)品分類(lèi)
載體基本信息
| Promega |
| pSI載體 |
| 哺乳動(dòng)物細(xì)胞表達(dá)載體 |
| CMV |
| 高 |
| 多克隆位點(diǎn)，限制性?xún)?nèi)切酶 |
| 3632 bp |
| SV40 pro-F: 5'-TATTTATGCAGAGGCCGAGG-3' |
| EBV-R: 5'-GTGGTTTGTCCAAACTCATC-3' |
| / |
| 氨芐 |
| / |
/ |
| E1721 |
| 穩(wěn)表達(dá)/ 瞬表達(dá) |
| 組成型 Constitutive |
| 非病毒 |
載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
載體簡(jiǎn)介
PCI和PSI 哺乳動(dòng)物表達(dá)載體，旨在提升哺乳動(dòng)物細(xì)胞中插入的克隆DNA基因的組成型表達(dá)。這兩個(gè)載體之間的差異在于增強(qiáng)子/啟動(dòng)子區(qū)域，該區(qū)域能夠控制插入基因的表達(dá)水平。 PSI表達(dá)載體包含猿猴病毒40（SV40）增強(qiáng)子和早期啟動(dòng)子區(qū)域（early promoter region），而PCI表達(dá)載體含有人巨細(xì)胞病毒（CMV）的主要及時(shí)早期基因增強(qiáng)子/啟動(dòng)子區(qū)域（immediate-early gene enhancer/promoter region）。
PSI和PCI載體可用于哺乳細(xì)胞的瞬時(shí)和穩(wěn)定基因表達(dá)。對(duì)于哺乳細(xì)胞基因的穩(wěn)表達(dá)，PSI或PCI載體必須共轉(zhuǎn)染一個(gè)可篩選的表達(dá)載體。
載體序列
LOCUS pSI 3632 bp DNA circular SYN
DEFINITION pSI
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..3632
/organism="pSI"
/mol_type="other DNA"
misc_feature complement(79..99)
/label="pBABE_3_primer"
misc_feature complement(85..300)
/label="SV40_enhancer"
promoter 97..365
/label="SV40_promoter"
rep_origin 264..341
/label="SV40_origin"
misc_feature 326..345
/label="SV40pro_F_primer"
misc_feature 483..615
/label="chim_int"
promoter 660..678
/label="T7_promoter"
terminator complement(743..862)
/label="SV40_PA_terminator"
misc_feature complement(757..776)
/label="EBV_rev_primer"
rep_origin 1065..1371
/label="f1_origin"
misc_feature 1689..1711
/label="pGEX_3_primer"
promoter 1870..1898
/label="AmpR_promoter"
gene 1940..2800
/label="Ampicillin"
/gene="Ampicillin"
CDS 1940..2800
/label="ORF frame 2"
rep_origin 2955..3574
/label="pBR322_origin"
ORIGIN
1 GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG
61 GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC
121 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT
181 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
241 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
301 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG
361 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG
421 ATTCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG TTGGTCGTGA GGCACTGGGC
481 AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA
541 GACAGAGAAG ACTCTTGCGT TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG
601 CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT
661 AATACGACTC ACTATAGGCT AGCCTCGAGA ATTCACGCGT GGTACCTCTA GAGTCGACCC
721 GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT
781 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
841 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
901 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA
961 ATCGATAAGG ATCCGGGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA
1021 CAGTTGCGCA GCCTGAATGG CGAATGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
1081 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
1141 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
1201 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
1261 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA
1321 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC
1381 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA
1441 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA
1501 TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG
1561 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC
1621 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
1681 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG
1741 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
1801 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
1861 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
1921 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT
1981 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
2041 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
2101 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
2161 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
2221 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
2281 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
2341 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
2401 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
2461 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
2521 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT
2581 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG
2641 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC
2701 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA
2761 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC
2821 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT
2881 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
2941 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
3001 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
3061 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
3121 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
3181 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
3241 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
3301 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
3361 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
3421 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
3481 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
3541 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
3601 CTGGCCTTTT GCTCACATGG CTCGACAGAT CT
//
DEFINITION pSI
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..3632
/organism="pSI"
/mol_type="other DNA"
misc_feature complement(79..99)
/label="pBABE_3_primer"
misc_feature complement(85..300)
/label="SV40_enhancer"
promoter 97..365
/label="SV40_promoter"
rep_origin 264..341
/label="SV40_origin"
misc_feature 326..345
/label="SV40pro_F_primer"
misc_feature 483..615
/label="chim_int"
promoter 660..678
/label="T7_promoter"
terminator complement(743..862)
/label="SV40_PA_terminator"
misc_feature complement(757..776)
/label="EBV_rev_primer"
rep_origin 1065..1371
/label="f1_origin"
misc_feature 1689..1711
/label="pGEX_3_primer"
promoter 1870..1898
/label="AmpR_promoter"
gene 1940..2800
/label="Ampicillin"
/gene="Ampicillin"
CDS 1940..2800
/label="ORF frame 2"
rep_origin 2955..3574
/label="pBR322_origin"
ORIGIN
1 GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG
61 GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC
121 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT
181 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
241 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
301 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG
361 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG
421 ATTCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG TTGGTCGTGA GGCACTGGGC
481 AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA
541 GACAGAGAAG ACTCTTGCGT TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG
601 CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT
661 AATACGACTC ACTATAGGCT AGCCTCGAGA ATTCACGCGT GGTACCTCTA GAGTCGACCC
721 GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT
781 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
841 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
901 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA
961 ATCGATAAGG ATCCGGGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA
1021 CAGTTGCGCA GCCTGAATGG CGAATGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
1081 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
1141 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
1201 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
1261 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA
1321 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC
1381 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA
1441 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA
1501 TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG
1561 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC
1621 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
1681 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG
1741 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
1801 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
1861 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
1921 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT
1981 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
2041 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
2101 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
2161 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
2221 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
2281 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
2341 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
2401 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
2461 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
2521 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT
2581 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG
2641 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC
2701 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA
2761 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC
2821 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT
2881 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
2941 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
3001 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
3061 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
3121 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
3181 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
3241 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
3301 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
3361 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
3421 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
3481 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
3541 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
3601 CTGGCCTTTT GCTCACATGG CTCGACAGAT CT
//
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pcDNA3.1/GS
pcDNA3.1/Zeo(+)
pcDNA3.1/Zeo(-)
pIREShyg3
pIRESpuro3
pGenesil-1
pBK-CMV
pNTAP-B
pVAX1
pIRESneo
pIRESneo2
pSI
pCI
pcDNA3.1/Hygro(+)
pBI-CMV1
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