BACKBONE

• Vector backbone
  pCLXSN
  (Search Vector Database)
• Backbone manufacturer
  Addgene Plasmid 12343 (Dr. Inder Verma)
• Backbone size (bp)7807
• Vector type
  Mammalian Expression, Retroviral

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

• Gene/Insert name
  None
• Tag / Fusion Protein
  • GFP (C terminal on backbone)

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ sequencing primerpLXSN 5
(Common Sequencing Primers)

RESOURCE INFORMATION

• A portion of this plasmid was derived from a plasmid made by
  PCLXSN-based plasmids were constructed using the pCLXSN vector obtained from Dr. Inder Verma.
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials