BACKBONE

• Vector backbone
  pOZ-FH-C
  (Search Vector Database)
• Backbone manufacturer
  Created by Yoshihiro Nakatani

• Backbone size (bp)7900

• Modifications to backbone
  pOZ-FH-C-puro vector was made by modifying the pOZ-FH-C vector (Nakatani and Ogryzko, 2003). A PCR-amplified puromycin resistance gene (isolated from pBabe-puro) was cloned in place of interleukin-2Rα using NcoI and BamHI site.
• Vector type
  Mammalian Expression, Retroviral
• Selectable markers
  Puromycin

• Tag / Fusion Protein

  • FLAG and HA (C terminal on backbone)

  
  

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

RESOURCE INFORMATION

• Terms and Licenses

  • UBMTA