BACKBONE

• Vector backbone
  pMIEG3
  (Search Vector Database)
• Backbone manufacturer
  David A. Williams (PMID: 10961859)
• Modifications to backbone
  IRES EGFP of pMIEG replaced with human CD4 cDNA
• Vector type
  Mammalian Expression, Retroviral
• Tag / Fusion Protein
  • IRES hCD4 (C terminal on backbone)

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (2)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ sequencing primerpLXSN_5
• 3′ sequencing primerIRES-R2 (gacggcaatatggtggaaa)
(Common Sequencing Primers)

RESOURCE INFORMATION

• A portion of this plasmid was derived from a plasmid made by
  Cloned human CD4 cDNA provided by G. Alkhatib, Indiana University.
• Terms and Licenses
  • UBMTA