pBABE-hTERT plasmid

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pBABE-hTERT細胞永生化載體逆病毒表達質粒，Amp抗性，Neo-G418細胞篩選標記。

Map圖譜

Retroviralexpression of hTERT. Used to create immortalized cell lines.

• Vector     backbone pBABE-neo

• Backbone size w/o insert (bp)5330

• Vector type Mammalian     Expression, Retroviral

• Selectable     markers Neomycin (select with G418)

• Bacterial     Resistance(s) Ampicillin

• Growth     Temperature 37°C

• Growth     Strain(s) DH5alpha

• Copy number High     Copy

• Gene/Insert     name hTERT

• Alt name telomerase     reverse transcriptase

• Species H.     sapiens (human)

• Insert Size     (bp) 3500

• Entrez Gene

TERT(a.k.a.CMM9, DKCA2, DKCB4, EST2,PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

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  Cloning methodRestriction     Enzyme

  
  

• 5′ cloning siteEcoRI     (not destroyed)

• 3′ cloning siteSalI     (not destroyed)

• 5′ sequencing primerpBABE     5'

• 3′ sequencing primerpBABE     3'