• Vector backbone
  pAAV
  (Search Vector Database)

• Backbone size w/o insert (bp)2905

• Total vector size (bp)5314

• Vector type
  Mammalian Expression, Mouse Targeting, AAV, CRISPR

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

GENE/INSERT 1

• Gene/Insert name
  U6_(SpaI)_sgRNA
• Species
  Synthetic
• Insert Size (bp)
  351

• PromoterU6

CLONING INFORMATION FOR GENE/INSERT 1

• Cloning methodGibson Cloning

• 5′ sequencing primercgcgtgagggcctatttcc

• 3′ sequencing primergcaagtgggttttaggaccag

GENE/INSERT 2

• Gene/Insert name
  Syn_EGFP-KASH
• Species
  Synthetic
• Insert Size (bp)
  349

• PromoterSynapsin

CLONING INFORMATION FOR GENE/INSERT 2

• Cloning methodGibson Cloning

• 5′ sequencing primerctgcgtatgagtgcaagtgggtt

• 3′ sequencing primerccgttggtgtaccgcagcatg