• Vector backbone
  pAAV
  
  

• Backbone size w/o insert (bp)2905

• Total vector size (bp)7400

• Vector type
  Mammalian Expression, AAV, CRISPR

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

GENE/INSERT

• Gene/Insert name
  SpCas9
• Alt name
  S. pyogenes Cas9 nuclease
• Alt name
  Cas9
• Species
  Synthetic
• Insert Size (bp)
  4200

• PromoterpMecp2

• Tag / Fusion Protein

  • HA (N terminal on insert)

  
  

CLONING INFORMATION

• Cloning methodRestriction Enzyme

• 5′ cloning siteAgeI (not destroyed)

• 3′ cloning siteEcoRI (not destroyed)

• 5′ sequencing primerAATGGGGTCCGCCTCTTTTCC

• 3′ sequencing primercttagctggcctccacctttctcttc