• Vector backbone
  pMyc-CMV
  
  
• Backbone manufacturer
  Clontech

• Backbone size w/o insert (bp)3791

• Vector type
  Mammalian Expression

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Growth instructions
  DH5 alpha
• Copy number
  High Copy

GENE/INSERT

• Gene/Insert name
  VN155(I152L)
• Alt name
  Venus (1-154, I152L)
• Species
  Aequorea victoria
• Insert Size (bp)
  462
• Mutation
  I152L

• Tag / Fusion Protein

  • Myc (N terminal on backbone)

  
  

CLONING INFORMATION

• Cloning methodRestriction Enzyme

• 5′ cloning siteKpnI (not destroyed)

• 3′ cloning siteNotI (not destroyed)