pGL4.31[luc2P-Gal4UAS-Hygro]轉錄激活報告載體

BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

Luc2P熒光報告基因Luciferase，GAL4UAS結合序列，Amp抗性，Hygromycin潮霉素哺乳動物細胞篩選標記。

The pGL4.31[luc2P/Gal4 UAS/Hygro] Vector(a,b) is designed to report transcriptional activation using the

firefly luciferase reporter gene. This reporter vector contains five consensus binding sequences, or Upstream Activating

Sequences, for the Gal4 DNA-binding domain (Gal4 UAS) and a minimal adenoviral promoter upstream of the firefly

luciferase gene. The vector backbone is based on the pGL4 luciferase reporter vector series, which has been engineered to

reduce the occurrence of anomalous transcription. The luc2P reporter gene is a synthetic firefly luciferase sequence, which

has been codon-optimized for high mammalian expression and contains minimal cryptic DNA regulatory elements. In

addition, the luc2P gene incorporates a protein degradation sequence, PEST, resulting in a destabilized reporter protein with

a faster response to transcriptional regulation. The vector also contains an ampicillin selection marker (Amp) for E. coli and a

hygromycin selection marker (Hygro) for mammalian cells. The pGL4.31[luc2P/Gal4 UAS/Hygro] Vector is provided for use

with the CheckMate?/Flexi? Vector Mammalian Two-Hybrid System

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