The pGL4.15[luc2P/Hygro] Vector(a–d) encodes the luciferase reporter gene luc2P (Photinus pyralis) and is

designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker

for hygromycin resistance in which the number of transcription factor-binding sites has been reduced and mammalian codon

usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a

synthetic reporter gene that is codon-optimized for mammalian expression.

The pGL4.15[luc2P/Hygro] Vector is a basic vector with no promoter. However, the vector contains a multiple cloning region

to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The

protein encoded by luc2P responds more quickly and with a greater magnitude to changes in transcriptional activity than the

luc2 gene, its more stable counterpart.

For easy transfer from one pGL4 Vector to another, the multiple cloning region is consistent throughout the pGL4 Vector

series. For easy transfer between pGL3 Vectors and pGL4 Vectors, many of the restriction enzyme sites present in the

pGL3 Vectors are also present in the pGL4 Vectors.

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