RK13 cell line兔腎細(xì)胞株

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

Organism:Oryctolagus
cuniculus, rabbit /
Tissue:kidney /
Disease:normal

Cat No.: NTCCL-37

Organism	Oryctolagus cuniculus, rabbit
Tissue	kidney
Product Format	frozen
Morphology	epithelial
Culture    Properties	adherent
Biosafety    Level	2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)] Genes    Expressed keratin Cellular    Products keratin Virus    Susceptibility Herpes    simplex virus    Pseudorabies virus    Vaccinia virus    Rabbitpox virus    Myxoma virus    Simian adenovirus 11    Simian adenovirus 17    Simian adenovirus 3    Simian adenovirus 1    Simian adenovirus 20    Simian adenovirus 18    Simian adenovirus 16    Simian adenovirus 8    Simian adenovirus 19    Simian adenovirus 21    Simian adenovirus 25    Simian adenovirus 22    Simian adenovirus 23    Simian adenovirus 38    Simian adenovirus 37    Simian adenovirus 27    Simian adenovirus 27    Simian adenovirus 39    Simian adenovirus 32    Simian adenovirus 34    Simian adenovirus 31    Simian adenovirus 33    Simian adenovirus 36    Rubella virus , Rubella virus Comments The cells    are positive for keratin by immunoperoxidase staining. The cells are    positive for bovine viral diarrhea virus (BVDV).
Disease	normal
Age	5 weeks
Applications	This cell line is a suitable transfection host.
Complete    Growth Medium	The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25%    (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which  contains trypsin inhibitor. 3. Add 2.0 to 3.0 mL of Trypsin-EDTA    solution to flask and observe cells under an inverted microscope until cell    layer is dispersed (usually within 5 to 15 minutes).    Note: To avoid clumping do not agitate the cells by hitting or shaking the    flask while waiting for the cells to detach. Cells that are difficult to    detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 mL of complete growth    medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell    suspension to new culture vessels. 6. Incubate cultures at 37°C. Subcultivation    Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium    Renewal: Twice per week
Cryopreservation	Freeze medium: culture    medium, 95%; DMSO, 5% Storage    temperature: liquid nitrogen vapor phase
Culture    Conditions	Atmosphere: air,    95%; carbon dioxide (CO2), 5% Temperature: 37°C
References	Didier ES, et    al. Characterization of Encephalitozoon (Septata) intestinailis isolates    cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS    patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 Beale AJ, et    al. Rabbit Cells Susceptible to Rubella Virus. The Lancet 282: 640-641 1963 Bolin SR, et    al. Survey of cell lines in the American Type Culture Collection for bovine    viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438