BL21-Gold(DE3) 大腸桿菌菌株-感受態(tài)細胞-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

BL21 and BL21-Gold-derived competent cells aredesigned for high-level protein expression using T7 RNA polymerase-basedexpression systems.The BL21(DE3) and BL21-Gold(DE3) competent cells areall-purpose strains for high-level protein expression and easy induction. Whenused with the CE6 bacteriophage, the BL21 and BL21-Gold cells provide thetightest control of protein expression, important for extremely toxic proteins.In addition, the BL21 and BL21-Gold cells can be used for non-T7 RNA polymeraseprotein expression systems. All BL21 strains are deficient in the OmpT and Lonproteases, which may interfere with isolation of intact recombinant proteins.Use the BL21 or the BL21-Gold competent cells and its derivatives with T7promoter-driven vectors, such as the Affinity? pCAL vectors and pET vectors.

Genotype E. coli B F- dcm+ Hte ompT hsdS(r_B- m_B-) gal λ (DE3) endA Tet^r