Cell line: JURKAT

Species:

human (Homo sapiens)

Cell type:

T cell leukemia

Origin:

established from the peripheral blood of a 14-year-oldboy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; oftenthis cell line is called "JM" (JURKAT and JM are derived from thesame patient and are sister clones), occasionally JM may be a subclone withsomewhat divergent features

Biosafety level:

1

Permissions and restrictions:

A

NTCC Cell Culture Data:

Morphology:

round cells growing singly or in clumps in suspension;image;image

Medium:

90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine

Subculture:

optimal split ratio of about 1:2 to 1:3 every 2-3 days;seed out after thawing at about 1 x 10⁶cells/ml; maintain at 0.5-1.5 x 10⁶cells/ml

Incubation:

at 37 °C with 5% CO₂

Doubling time:

about 25-35 hours

Harvest:

maximal density at about 1.5 x 10⁶cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:

Mycoplasma:

contamination was eliminated with Mycoplasma RemovalAgent, then negative in DAPI, microbiological culture, RNA hybridization, PCRassays

Immunology:

CD2 +, CD3 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -,TCRalpha/beta +, TCRgamma/delta -;image

Fingerprint:

multiplex PCR of minisatellite markers revealed a uniqueDNA profile

Species:

confirmed as human with IEF of AST, LDH, NP

Cytogenetics:

human flat-moded hypotetraploid karyotype with 7.8%polyploidy - 87(78-91)<4n>XX, -Y, -Y, -5, -16, -17, -22,add(2)(p21)/del(2)(p23)x2 - sideline with additional der(5)t(5;10)(q11;p15),del(9)(p11)

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -,HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -