Rhizobium sp. R1000發(fā)根農(nóng)桿菌菌株/感受態(tài)細(xì)胞

-BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

Cat No.: NTCC543056

Designation: R1000

Deposited Name: Agrobacterium rhizogenes (Riker et al.) Conn

Product Description: Formerly known as Rhizobium rhizogenes.

Medium

Medium 3: Nutrient agar or nutrient broth

Growth Conditions

Temperature: 26° C

Atmosphere: Aerobic

Propagation Procedure

1. Open vial according to enclosed instructions.

2. From a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette and use to

rehydrate the entire pellet.

3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.

4. Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or plate.

5. Incubate the tubes and plate at 26° C for 24 to 48 hours.

Comments:

Colonies on #3 agar are cream, circular, entire, mucoid, convex, and translucent.