• Vector backbone
  pcDNA3.1
  (Search Vector Database)

• Backbone size w/o insert (bp)5354

• Total vector size (bp)9400

• Vector type
  Mammalian Expression, CRISPR
• Selectable markers
  Neomycin (select with G418)

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Copy number
  Unknown

GENE/INSERT

• Gene/Insert name
  hPdCpf1
• Alt name
  Cpf1
• Species
  Prevotella_disiens
• Insert Size (bp)
  4107

• PromoterCMV

• Tag / Fusion Protein

  • NLS & 3xHA (C terminal on insert)

  
  

CLONING INFORMATION

• Cloning methodUnknown

• 5′ sequencing primerCMV-F

• 3′ sequencing primerBGH-rev