pRedCas9 plasmid 71541

-BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

pRedCas9大腸桿菌CRISPR/Cas9介導(dǎo)的可誘導(dǎo)型Red重組系統(tǒng)基因組編輯質(zhì)粒，組成型表達(dá)Cas9，誘導(dǎo)型表達(dá)Red重組系統(tǒng)，誘導(dǎo)型表達(dá)質(zhì)粒curing系統(tǒng)，可用于CRISPR介導(dǎo)的大腸桿菌基因組編輯。

Map圖譜：

Description:

Constitutiveexpression of Cas9, inducible expression of the Red recombineering system, andinducible expression of the plasmid curing system for CRISPR mediated genomeediting of E. coli.

• 
  

• Vector backbone

pSC101ts

• Vector type

Bacterial Expression

• Bacterial Resistance(s)

Spectinomycin

• Growth Temperature

30°C

• Growth Strain(s)

DH5alpha

GENE/INSERT1

• Gene/Insert name

Cas9

• Species

S. pyogenes

GENE/INSERT2

• Gene/Insert name

lambda red genes

CLONINGINFORMATION FOR GENE/INSERT 2

• 5′ sequencing primerM13pUC-Rev

GENE/INSERT3

• Gene/Insert name

plasmid curing system

• Alt name

gRNA targeting the bla gene

• Promoter  pBAD

• 5′ sequencing primerpBAD-F