• Vector backbone
  pSIN4-EF2-IRES-Pur
  
  

• Backbone size w/o insert (bp)5500

• Vector type
  Mammalian Expression, Lentiviral ; reprogramming

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  Low Copy

GENE/INSERT

• Gene/Insert name
  Klf4, cMyc
• Species
  H. sapiens (human)
• Insert Size (bp)
  2733
• GenBank ID
  NM_004235.2NM_002467
• Entrez Gene
  MYC(a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

CLONING INFORMATION

• Cloning methodRestriction Enzyme

• 5′ cloning siteNheI (not destroyed)

• 3′ cloning siteSpeI (not destroyed)

• 5′ sequencing primerEF1a_for