BioVector? ATCC 33559 結(jié)腸彎曲桿菌標(biāo)準(zhǔn)質(zhì)控株

BioVector? ATCC 33559 Campylobacter coli Quality Control Strain

第一部分 中文說明

一 產(chǎn)品基本信息與遺傳學(xué)背景

• 菌種名稱 BioVector? ATCC 33559 結(jié)腸彎曲桿菌（大腸彎曲桿菌）

• 別名與同義詞 Campylobacter coli (Doyle) Veron and Chatelain；同型異名 Vibrio coli。

• 分類學(xué)地位 細(xì)菌域（Bacteria），變形菌門（Pseudomonadota），彎曲菌屬（Campylobacter）。

• 保藏機(jī)構(gòu)菌株號 ATCC 33559 = CIP 70.80 = DSM 4689 = NCTC 11366。

• 分離來源與菌株性質(zhì)

  • 原始分離自豬糞便（Pig feces）。

  • 該菌株是經(jīng)國際公認(rèn)的全基因組測序標(biāo)準(zhǔn)模式物種菌株（Type strain）。

• 生物安全級別 2級（Risk Group 2，主要引起食源性胃腸炎）。

二 生理生化特征與特殊培養(yǎng)環(huán)境

• 形態(tài)學(xué)特征 顯微鏡下表現(xiàn)為革蘭氏陰性（Gram-negative）、能運(yùn)動、呈細(xì)長彎曲或微螺旋狀、逗點(diǎn)狀的桿菌。

• 氣體生長環(huán)境 屬于嚴(yán)格的微需氧菌（Microaerophilic）。在進(jìn)行體外人工培養(yǎng)時，必須在微需氧（通常為 5% 氧氣、10% 二氧化碳、85% 氮?dú)猓┑臍怏w生成包或厭氧罐中孵育。

• 推薦標(biāo)準(zhǔn)培養(yǎng)基

  • 液體基礎(chǔ) ATCC 富集肉湯培養(yǎng)基（如 #1115 培養(yǎng)基）。

  • 固體平板 彎曲菌選擇性血瓊脂平板（如 #260 瓊脂平板或巧克力瓊脂平板）。

• 物理培養(yǎng)參數(shù) 37攝氏度恒溫培養(yǎng) 48到72小時（所有試管或斜面在微需氧罐中應(yīng)保持瓶蓋松開狀態(tài)以利于氣體交換）。

三 凍干菌種復(fù)蘇與傳代標(biāo)準(zhǔn)步驟

1. 菌株復(fù)蘇操作

   • 無菌條件下打開包含凍干菌種的西林瓶。

   • 用無菌吸管吸取約 0.5 到 1.0 mL 預(yù)熱的完全肉湯培養(yǎng)基，注入凍干管中，輕輕吹吸使菌塊完全溶解重懸。

   • 將溶解后的菌懸液全部接種到 5 到 6 mL 的液體富集肉湯試管中，同時吸取數(shù)滴菌液涂布接種到血瓊脂平板或瓊脂斜面上。

2. 培養(yǎng)與保藏

   • 迅速將接種完成的試管和平板放進(jìn)微需氧裝置中，37攝氏度孵育 48小時。

   • 注：復(fù)蘇后的前48小時內(nèi)，切勿頻繁打開密封培養(yǎng)皿或厭氧罐，以避免因氧氣濃度波動導(dǎo)致脆弱的彎曲菌死亡。

   • 長期保藏 推薦使用含有 10% 滅菌甘油的保護(hù)劑，置于液氮或 -80攝氏度超低溫冰箱中冷凍保存。

四 核心科研與工業(yè)應(yīng)用方向

1. 微生物實(shí)驗(yàn)室質(zhì)量控制（QC Organism） BioVector? ATCC 33559 作為標(biāo)準(zhǔn)的彎曲菌模式菌株，被廣泛用作商業(yè)化培養(yǎng)基性能測試、革蘭氏染色試劑質(zhì)控、彎曲菌微量生化鑒定試劑盒（如 API 系統(tǒng)）的陽性對照標(biāo)準(zhǔn)株。

2. 食品安全與腸道傳染?。ㄊ吃葱灾虏【┭芯?用于建立針對食品（如家禽肉類、畜產(chǎn)品）中結(jié)腸彎曲桿菌殘留的標(biāo)準(zhǔn)前增殖、富集檢測方法（依據(jù) FDA BAM 或 ISO 11133 標(biāo)準(zhǔn)規(guī)范）。

3. 分子診斷與基因擴(kuò)增檢測標(biāo)準(zhǔn)品 該菌株提取的定量基因組 DNA（如 33559DQ 分子標(biāo)準(zhǔn)品）作為金標(biāo)準(zhǔn)線，廣泛用于多重實(shí)時熒光定量 PCR（qPCR）、下一代測序（NGS）等臨床分子診斷測定法的研發(fā)、驗(yàn)證與 Lot-to-Lot 穩(wěn)定性監(jiān)控。

PART 2 ENGLISH SECTION

I General Information and Genetic Background

• Culture Name BioVector? ATCC 33559 Campylobacter coli (Doyle) Veron and Chatelain

• Synonyms Homotypic synonym Vibrio coli Doyle 1948.

• Taxonomic Classification Domain Bacteria, Phylum Pseudomonadota, Genus Campylobacter, Species Campylobacter coli.

• Repositories Identifiers ATCC 33559 = CIP 70.80 = DSM 4689 = NCTC 11366.

• Isolate History and Strain Nature

  • Originally isolated from pig feces.

  • Serves as a whole-genome sequenced bacterial type strain recognized globally.

• Biosafety Level BSL-2 (Risk Group 2 due to its enteric pathogenicity).

II Morphological Attributes and Cultivation Media

• Morphology Reveals classic Gram-negative, motile, curved, comma-shaped, or slender helical rod profiles under oil-immersion microscopy.

• Growth Atmosphere Requires strict microaerophilic environments to proliferate. Standard culture should occur under controlled gas layers (ideally 5% O2, 10% CO2, and 85% N2).

• Standard Growth Media Recipes

  • Basal Liquid Broth ATCC Enrichment Medium (e.g., #1115 Formulation).

  • Solid Agar Campylobacter selective Blood Agar plates (or Chocolate Agar formulations).

• Physical Incubation Thresholds Incubated closely at 37 degrees Celsius for 48 to 72 hours, maintaining loose caps inside microaerophilic jars to ensure seamless gas exchange tracks.

III Rehydration and Maintenance Protocols

1. Standard Pelleted Culture Recovery

   • Aseptically unpack the lyophilized vial inside a certified bio-safety hood.

   • Rehydrate the dried disc pellet by injecting 0.5 to 1.0 mL of pre-warmed broth base directly into the ampoule, mixing gently until thoroughly dissolved.

   • Inoculate the mixture into a secondary test tube carrying 5 to 6 mL of fresh liquid medium and streak several drops across blood agar plates or slants.

2. Continuous Preservation Parameters

   • Promptly deposit the fresh culture matrix inside a microaerophilic container and run incubation at 37 degrees Celsius for 48 hours.

   • Tip: Avoid opening the closed agar plates or venting the anaerobic jar during the preliminary 48-hour cycle to shield sensitive cells from oxygen shock.

   • Long-term Storage Cryopreservation is achieved at liquid nitrogen thresholds or in a -80 degrees Celsius freezer using 10% sterile glycerol cryoprotectant arrays.

IV Strategic Research and Quality Control Applications

1. Microbiology Laboratory Quality Control (QC Organism Reference) BioVector? ATCC 33559 is widely used for media performance evaluations, validating Gram stain accuracy, confirming identification kits, and auditing standard clinical bacteriological procedures.

2. Food Safety and Enteric Pathogen Screening Deployed to standardise enrichment metrics and validate pathogen screening methods from meat matrices following official FDA BAM or ISO 11133 compliance rules.

3. Molecular Standards Development and Diagnostic Assays Purified quantitative genomic DNA derived from this specific clone (e.g., ATCC 33559DQ standards) is utilized to generate exact standard curves for qPCR or validate NGS pipelines.

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