BioVector? WTC-11 (WTC) 人誘導(dǎo)多能干細(xì)胞 / BioVector? WTC-11 Human Induced Pluripotent Stem Cell Line

通用定義 / General Definition:BioVector? WTC-11（通常簡稱為 WTC）是一種具有高度科研價(jià)值的人源誘導(dǎo)多能干細(xì)胞 (iPSC) 系。該細(xì)胞系由加州大學(xué)舊金山分校（UCSF）的 Bruce Conklin 實(shí)驗(yàn)室建立，通過利用 episomal 質(zhì)粒將人類男性皮膚成纖維細(xì)胞重編程而成。WTC-11 被選為 Allen 細(xì)胞科學(xué)研究所 (Allen Institute for Cell Science) 的核心基準(zhǔn)細(xì)胞系，用于構(gòu)建一系列熒光標(biāo)記的細(xì)胞器轉(zhuǎn)基因株系。它以核型穩(wěn)定、分化效率高以及對 CRISPR/Cas9 基因編輯具有極高的耐受性和效率而著稱，是全球細(xì)胞生物學(xué)、類器官建模和系統(tǒng)生物學(xué)研究的“金標(biāo)準(zhǔn)”工具。

BioVector? WTC-11 (often referred to as WTC) is a highly characterized human induced pluripotent stem cell (iPSC) line. Established by the laboratory of Bruce Conklin at UCSF, it was generated by reprogramming human male skin fibroblasts using non-integrating episomal plasmids. WTC-11 was selected as the foundational line for the Allen Institute for Cell Science, where it serves as the parent for a vast collection of fluorescently tagged organelle reporter lines. Renowned for its stable karyotype, high differentiation efficiency, and exceptional amenability to CRISPR/Cas9 gene editing, WTC-11 is a "gold standard" tool in cell biology, organoid modeling, and systems biology.

BioVector? WTC-11 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? WTC-11 人誘導(dǎo)多能干細(xì)胞

• 來源組織： 成年男性皮膚成纖維細(xì)胞 (Skin Fibroblasts)

• 重編程方式： 非整合型 Episomal 質(zhì)粒

• 核型： 正常 46, XY

• 主要特點(diǎn)： 基因組高度背景化，特別適合大規(guī)模成像和精準(zhǔn)基因編輯。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? mTeSR Plus 或 Essential 8 (E8)。

• 基質(zhì)支持： 建議使用 Matrigel 或 hESC-qualified Laminin-511 進(jìn)行無滋養(yǎng)層培養(yǎng)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2。

• 傳代建議： 建議在匯合度達(dá)到 70-80% 時(shí)進(jìn)行傳代，使用 EDTA (Versene) 進(jìn)行團(tuán)塊傳代。

3. 細(xì)胞應(yīng)用

• 高內(nèi)涵成像： 作為 Allen 細(xì)胞庫的親本株，用于觀察亞細(xì)胞結(jié)構(gòu)動(dòng)力學(xué)。

• 心肌分化： WTC-11 在分化為功能性心肌細(xì)胞（iPSC-CMs）方面具有極高的重現(xiàn)性。

• 疾病建模： 利用 CRISPR 技術(shù)引入致病突變，通過同源基因背景研究表型。

4. 注意事項(xiàng)

• 單細(xì)胞克?。?/strong> 雖然 WTC-11 對單細(xì)胞操作耐受良好，但在克隆篩選時(shí)必須添加 ROCK 抑制劑 (Y-27632) 以維持成活。

• 分化監(jiān)測： 定期檢測 SSEA-4 和 Tra-1-60 等表面標(biāo)記物，確保群體的多能性維持在 95% 以上。

English Datasheet

1. General Product Information

• Product Name: BioVector? WTC-11 Human Induced Pluripotent Stem Cell Line

• Source Tissue: Adult male skin fibroblasts

• Reprogramming Method: Non-integrating Episomal plasmids

• Karyotype: Stable 46, XY

• Distinction: The primary line used by the Allen Institute for creating the Integrated Cell collection.

2. Culture Conditions

• Basal Medium: BioVector? mTeSR Plus or Essential 8 (E8) defined media.

• Support Matrix: Feeder-free culture on Matrigel or Laminin-511 (iMatrix-511).

• Incubation: 37°C, 5% CO2.

• Passaging: Clump passaging using EDTA (Versene) is recommended; typically subcultured every 3-4 days at 1:10 to 1:20 ratios.

3. Applications

• Live-Cell Imaging: Ideal for quantitative analysis of cell architecture and organelle interactions.

• Cardiomyocyte Differentiation: Widely recognized as one of the most efficient lines for producing high-purity cardiomyocytes for drug screening.

• Precision Engineering: Optimized for seamless CRISPR-mediated knock-ins and knock-outs.

4. Key Usage Notes

• Genomic Integrity: WTC-11 is valued for its baseline genomic stability; however, regular G-band karyotyping or SNP arrays are advised for long-term cultures.

• Feeding Schedule: For E8 media, daily feeding is mandatory; for mTeSR Plus, weekend-free feeding protocols can be utilized.

注意 / Note: WTC-11 應(yīng)在生物安全二級 (BSL-2) 條件下操作。該細(xì)胞系具有極強(qiáng)的單細(xì)胞增殖能力，非常適合用于建立轉(zhuǎn)基因穩(wěn)定株。WTC-11 should be handled under Biosafety Level 2 (BSL-2) conditions. Due to its superior single-cell survival and proliferative capacity, it is an ideal platform for establishing transgenic stable lines.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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