BioVector? mND2-0 (MIRJT7i-mND2-0) 人誘導多能干細胞 / BioVector? mND2-0 Human Induced Pluripotent Stem Cell Line

通用定義 / General Definition:

BioVector? mND2-0（全稱 MIRJT7i-mND2-0）是一種人源誘導多能干細胞 (iPSC) 系。該細胞系最初由威斯康星大學麥迪遜分校的 James Thomson 實驗室建立，是利用非整合型質(zhì)粒載體（如 Episomal vectors）轉(zhuǎn)染人包皮成纖維細胞重編程而來的。mND2-0 是 NHLBI 祖細胞生物學聯(lián)合會 (PCBC) 核心基因組庫的重要組成部分。由于其具有高度的多能性和穩(wěn)定的基因組背景，它被廣泛用于人類器官構(gòu)建、干細胞定向分化研究（如耳蝸、前庭和皮膚類器官）以及高通量基因組學分析。

BioVector? mND2-0 (also known as MIRJT7i-mND2-0) is a human induced pluripotent stem cell (iPSC) line. Originally derived in the laboratory of James Thomson at the University of Wisconsin-Madison, it was generated by reprogramming human foreskin fibroblasts using non-integrating episomal vectors. mND2-0 is a key reference line within the NHLBI Progenitor Cell Biology Consortium (PCBC) collection. Due to its robust pluripotency and well-characterized genomic profile, it is extensively utilized in the generation of complex human organoids (such as cochlear, vestibular, and hair-bearing skin organoids) and large-scale functional genomics studies.

BioVector? mND2-0 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? mND2-0 人誘導多能干細胞

• 來源組織： 人新生兒包皮成纖維細胞 (Foreskin Fibroblasts)

  
  

• 重編程方式： Episomal 質(zhì)粒（含 OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4）

• 生物特性： 多能性 (Pluripotency)，核型通常為正常 46, XY

• 應用領域： 類器官誘導（內(nèi)耳、皮膚）、發(fā)育生物學模型、再生醫(yī)學研究

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? mTeSR Plus 或 TeSR-E8。

• 基質(zhì)支持： 建議使用 Matrigel 或 Vitronectin 進行無滋養(yǎng)層培養(yǎng)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2。

• 傳代比例： 1:6 至 1:10；建議采用團塊傳代法（如 EDTA 或 ReLeSR）以維持干性。

3. 細胞應用

• 類器官建模： 近年來多篇頂級文獻使用 mND2-0 成功誘導了具有功能性毛細胞的耳蝸類器官和帶有毛囊的皮膚類器官。

• 基因組學對照： 作為 PCBC 計劃的標準株，其全基因組測序、RNA-seq 及甲基化數(shù)據(jù)均可在公開數(shù)據(jù)庫查閱。

• 分化能力： 可定向分化為神經(jīng)元、心肌細胞及內(nèi)皮細胞，用于疾病建模。

4. 注意事項

• 干性維持： iPSC 對培養(yǎng)基質(zhì)量非常敏感。如果出現(xiàn)分化跡象（細胞邊緣變得平滑或出現(xiàn)非典型形態(tài)），需及時手動剔除。

• 存儲建議： 建議在液氮中長期保存，復蘇時使用 Y-27632 (ROCK inhibitor) 以提高細胞存活率。

English Datasheet

1. General Product Information

• Product Name: BioVector? mND2-0 Human Induced Pluripotent Stem Cell Line

  
  

• Parental Source: Human neonatal foreskin fibroblasts

  
  

• Reprogramming Method: Non-integrating Episomal vectors (chemically defined conditions)

  
  

• Biological Properties: Pluripotent; characteristically exhibits a stable 46, XY karyotype.

• Primary Use: Organoid generation (inner ear, skin), developmental modeling, stem cell standardization.

2. Culture Conditions

• Basal Medium: BioVector? mTeSR Plus or TeSR-E8.

• Support Matrix: Feeder-free culture using Matrigel or recombinant Vitronectin.

• Incubation: 37°C, 5% CO2.

• Subculturing: Passage every 4-6 days (1:6 to 1:10 ratio) using clump-dissociation reagents such as EDTA.

3. Applications

• High-Fidelity Organoids: A preferred line for generating vestibular and cochlear organoids, as well as complex hair-bearing skin organoids.

• Reference Standards: Included in the PCBC genomic resource, providing a benchmark for SNP array, DNA methylation, and transcriptomics analyses.

• Lineage Specification: Ideal for studying the molecular switches governing the transition from pluripotency to ectodermal and mesodermal lineages.

4. Key Usage Notes

• Morphology Monitoring: Colonies should be tightly packed with well-defined borders. Spontaneous differentiation should be monitored and physically removed to ensure culture purity.

• Recovery: The use of a ROCK inhibitor (e.g., Y-27632) for the first 24 hours post-thawing is essential to maximize the recovery of high-quality pluripotent clusters.

注意 / Note: mND2-0 應在生物安全二級 (BSL-2) 條件下操作。使用前建議通過免疫熒光檢測 OCT4, SSEA4 等標志物確認其多能狀態(tài)。

mND2-0 should be handled under Biosafety Level 2 (BSL-2) conditions. BioVector? recommends verifying pluripotency markers such as OCT4 and SSEA4 via immunofluorescence before initiating large-scale differentiation protocols.

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

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