BioVector? WA25 人類胚胎干細(xì)胞系 / BioVector? WA25 Human Embryonic Stem Cell Line

通用定義 / General Definition:BioVector? WA25（通常被稱為 H25）是一種源自人類的胚胎干細(xì)胞系 (hESC)。它是最早由威斯康星大學(xué)麥迪遜分校 James Thomson 實(shí)驗(yàn)室建立的一系列具有里程碑意義的細(xì)胞系之一。WA25 具有多能性 (Pluripotency)，這意味著在適當(dāng)?shù)呐囵B(yǎng)條件下，它可以無限增殖并分化為人體內(nèi)所有三個(gè)胚層（外胚層、中胚層和內(nèi)胚層）的任何細(xì)胞類型。該細(xì)胞系廣泛應(yīng)用于發(fā)育生物學(xué)、再生醫(yī)學(xué)、藥物篩選以及人類早期胚胎發(fā)育的研究。

BioVector? WA25 (commonly referred to as H25) is a human embryonic stem cell line (hESC). It is part of the original set of seminal cell lines established by James Thomson’s laboratory at the University of Wisconsin-Madison. WA25 is pluripotent, meaning that under appropriate culture conditions, it can proliferate indefinitely and differentiate into any cell type from the three germ layers (ectoderm, mesoderm, and endoderm). This cell line is extensively used in developmental biology, regenerative medicine, drug screening, and research into early human embryonic development.

BioVector? WA25 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? WA25 人胚胎干細(xì)胞

• 常用別名： H25

• 供體背景： 人類囊胚期胚胎

• 生物特性： 多能性 (Pluripotency)，核型通常為 46, XX（需核型鑒定確認(rèn)）

• 應(yīng)用領(lǐng)域： 分化誘導(dǎo)研究、干細(xì)胞自我更新機(jī)制、毒理學(xué)測試

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? mTeSR1 或 Essential 8 (E8) 無血清培養(yǎng)基。

• 基質(zhì)支持： 建議培養(yǎng)在 Matrigel 或 Vitronectin 涂層的培養(yǎng)皿上（無滋養(yǎng)層培養(yǎng)）。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2。

• 維持方式： 每 4 到 5 天傳代一次；建議使用 EDTA 或 Accutase 進(jìn)行溫和的團(tuán)塊傳代。

3. 細(xì)胞應(yīng)用

• 三胚層分化： 通過形成擬胚體 (Embryoid Bodies, EB) 或定向誘導(dǎo)，研究神經(jīng)、心肌或肝臟細(xì)胞的發(fā)育。

• 基因編輯： 利用 CRISPR/Cas9 系統(tǒng)研究特定基因?qū)S持多能性的作用。

• 藥物毒性評(píng)價(jià)： 在人類遺傳背景下評(píng)估候選藥物對早期發(fā)育的潛在毒性。

4. 注意事項(xiàng)

• 核型穩(wěn)定性： 胚胎干細(xì)胞在長期培養(yǎng)中可能出現(xiàn)染色體變異，建議每 10 到 15 代進(jìn)行一次核型分析。

• 自發(fā)分化： 需定期清除培養(yǎng)皿中出現(xiàn)的非典型分化克隆，以保持群體的純凈度。

English Datasheet

1. General Product Information

• Product Name: BioVector? WA25 Human Embryonic Stem Cell Line

• Alias: H25

• Source: Human blastocyst-stage embryo

• Biological Properties: Pluripotent; typically exhibits a 46, XX karyotype.

• Primary Use: Differentiation assays, stem cell self-renewal studies, regenerative medicine modeling.

2. Culture Conditions

• Basal Medium: BioVector? mTeSR1 or Essential 8 (E8) defined medium.

• Support Matrix: Feeder-free culture on Matrigel or Vitronectin coated surfaces.

• Incubation: 37°C, 5% CO2.

• Subculturing: Passage every 4-5 days using gentle dissociation reagents like EDTA or Accutase for clump-based passaging.

3. Applications

• Germ Layer Differentiation: Investigating the transition into ectoderm, mesoderm, or endoderm lineages via directed differentiation protocols.

• Regenerative Medicine: Developing protocols for generating functional human cells (e.g., neurons or cardiomyocytes) for cell-based therapies.

• Functional Genomics: A robust platform for studying the signaling pathways (such as Wnt, TGF-beta) that control human development.

4. Key Usage Notes

• Pluripotency Monitoring: Regularly verify the expression of pluripotency markers such as OCT4, NANOG, and SOX2 via immunofluorescence or qPCR.

• Media Quality: Always pre-warm media to room temperature (not 37°C for E8) to prevent premature differentiation during routine feeding.

注意 / Note: WA25 屬于人類胚胎干細(xì)胞，其使用必須嚴(yán)格遵守當(dāng)?shù)氐膫惱頊?zhǔn)則和法律法規(guī)。所有操作均需在生物安全二級(jí) (BSL-2) 條件下完成。WA25 is a human embryonic stem cell line; its use must strictly adhere to local ethical guidelines and regulations. All procedures must be performed under Biosafety Level 2 (BSL-2) conditions.

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