BioVector? JVE253 人類黑素瘤細(xì)胞系 / BioVector? JVE253 Human Melanoma Cell Line

通用定義 / General Definition:BioVector? JVE253 是一種源自人類的黑素瘤 (Melanoma) 細(xì)胞系。該細(xì)胞系主要用于皮膚腫瘤學(xué)、癌癥免疫學(xué)以及黑色素瘤生物學(xué)研究。JVE253 通常作為一種體外模型，用于探索黑色素瘤細(xì)胞的增殖、侵襲性、黑色素合成通路以及對(duì)各種化學(xué)療法和靶向藥物的敏感性。由于黑色素瘤具有高度的異質(zhì)性，JVE253 為研究特定基因突變背景下的腫瘤進(jìn)展提供了重要的細(xì)胞資源。

BioVector? JVE253 is a human-derived Melanoma cell line. This cell line is utilized primarily in dermatological oncology, cancer immunology, and basic melanoma biology research. JVE253 serves as an in vitro model for investigating melanoma cell proliferation, invasiveness, melanogenesis pathways, and sensitivity to various chemotherapeutic agents and targeted therapies. Given the high degree of heterogeneity in melanoma, JVE253 provides an important cellular resource for studying tumor progression within specific genetic mutation backgrounds.

BioVector? JVE253 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱: BioVector? JVE253 人黑色素瘤細(xì)胞

• 組織來源: 人類黑色素瘤組織

• 生長特性: 貼壁生長

• 細(xì)胞形態(tài): 典型的人類黑色素瘤細(xì)胞形態(tài)，通常表現(xiàn)為梭形或多角形。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基: BioVector? RPMI 1640 或 DMEM（取決于具體亞株需求）。

• 血清添加: 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 補(bǔ)充因子: 建議添加 2 mM L-谷氨酰胺和 1% 青霉素-鏈霉素溶液。

• 培養(yǎng)環(huán)境: 37 攝氏度，5% CO2。

• 傳代比例: 1:3 至 1:6，建議在匯合度達(dá)到 80% 到 90% 時(shí)進(jìn)行傳代。

3. 細(xì)胞應(yīng)用

• 腫瘤生物學(xué)研究: 黑色素瘤細(xì)胞周期調(diào)控、凋亡誘導(dǎo)及細(xì)胞遷移能力測(cè)試。

• 藥物篩選: 評(píng)估新型 BRAF 或 MEK 抑制劑等靶向藥物的抗腫瘤活性。

• 免疫學(xué)研究: 研究黑色素瘤細(xì)胞與免疫細(xì)胞（如 T 細(xì)胞、NK 細(xì)胞）之間的相互作用。

4. 注意事項(xiàng)

• 黑色素沉積: 該細(xì)胞在培養(yǎng)過程中可能會(huì)產(chǎn)生不同程度的黑色素，這可能影響培養(yǎng)基的顏色，屬于正常現(xiàn)象。

• 復(fù)蘇建議: 細(xì)胞復(fù)蘇后初期生長可能較慢，建議在復(fù)蘇后的前 24 到 48 小時(shí)內(nèi)不要頻繁移動(dòng)培養(yǎng)瓶。

English Datasheet

1. General Product Information

• Product Name: BioVector? JVE253 Human Melanoma Cell Line

• Source: Human melanoma tissue

• Growth Properties: Adherent

• Morphology: Characteristic melanoma morphology, typically appearing spindle-shaped or polygonal.

2. Culture Conditions

• Basal Medium: BioVector? RPMI 1640 or DMEM (depending on specific sub-line requirements).

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS).

• Supplements: 2 mM L-Glutamine and 1% Penicillin-Streptomycin are recommended.

• Incubation: 37 degrees Celsius, 5% CO2.

• Subculturing: 1:3 to 1:6 ratio; subculture when confluence reaches 80% to 90%.

3. Applications

• Cancer Research: Studying cell cycle regulation, apoptosis induction, and cell migration in melanoma.

• Drug Efficacy Assays: Testing the activity of novel targeted agents, such as BRAF or MEK inhibitors.

• Immune Interaction: Investigating the crosstalk between melanoma cells and immune effectors (e.g., T cells or NK cells).

4. Key Usage Notes

• Melanogenesis: The cells may produce varying levels of melanin during culture, which can darken the medium; this is a normal characteristic of this cell line.

• Thawing Procedure: Growth may be slow immediately after thawing. It is recommended to avoid excessive handling or observation during the first 24 to 48 hours post-recovery.

注意 / Note: JVE253 應(yīng)在生物安全二級(jí) (BSL-2) 條件下操作。在進(jìn)行特定的分子實(shí)驗(yàn)前，建議先確認(rèn)該細(xì)胞系的 BRAF, NRAS 等核心驅(qū)動(dòng)基因的突變狀態(tài)。JVE253 should be handled under Biosafety Level 2 (BSL-2) conditions. Before conducting specific molecular assays, it is recommended to verify the mutation status of key driver genes such as BRAF and NRAS for this cell line.

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