BioVector? H80X 抗人絨毛膜促性腺激素 alpha 雜交瘤細胞 / BioVector? H80X Anti-hCG alpha Hybridoma Cell Line

通用定義 / General Definition:BioVector? H80X 是一種專門用于產生單克隆抗體的小鼠雜交瘤細胞系。該細胞系通過將經過人絨毛膜促性腺激素 alpha 亞基 (hCG alpha) 免疫的小鼠脾細胞與小鼠骨髓瘤細胞融合而成。H80X 分泌的單克隆抗體能夠特異性識別 hCG 的 alpha 亞基。由于 hCG, LH, FSH 和 TSH 共享相同的 alpha 亞基，該細胞系生產的抗體常用于研究這類糖蛋白激素的共同結構特征，或在雙抗體夾心法 ELISA 中作為捕獲或檢測抗體對的一部分。

BioVector? H80X is a mouse hybridoma cell line dedicated to the production of monoclonal antibodies. It was generated by fusing splenocytes from mice immunized with the alpha subunit of Human Chorionic Gonadotropin (hCG alpha) with a mouse myeloma partner. The monoclonal antibody secreted by H80X specifically recognizes the alpha subunit of hCG. Since hCG, LH, FSH, and TSH all share a common alpha subunit, antibodies from this line are frequently used to study the shared structural motifs of these glycoprotein hormones or serve as part of a capture or detection pair in sandwich ELISA assays.

BioVector? H80X 技術說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產品基本信息

• 產品名稱: BioVector? H80X 抗 hCG alpha 雜交瘤細胞

• 抗體類型: 單克隆抗體 (mAb)

• 抗原特異性: 人絨毛膜促性腺激素 alpha 亞基 (hCG alpha)

• 宿主背景: Balb/c 小鼠

• 生長特性: 半懸浮生長

• 細胞形態(tài): 典型的雜交瘤形態(tài)，圓形，大小均勻

2. 培養(yǎng)條件

• 基礎培養(yǎng)基: BioVector? RPMI 1640

• 血清添加: 10% 到 15% BioVector? 優(yōu)質胎牛血清 (FBS)

• 補充因子: 2 mM L-谷氨酰胺，1 mM 丙酮酸鈉

• 培養(yǎng)環(huán)境: 37 攝氏度，5% CO2

• 傳代比例: 1:3 至 1:5，建議保持細胞密度在 200,000 到 1,000,000 cells/mL 之間

3. 細胞應用

• 抗體生產: 通過體外培養(yǎng)法或小鼠腹水法大規(guī)模生產抗 hCG alpha 單克隆抗體。

• 免疫檢測開發(fā): 用于開發(fā)早孕診斷試劑盒或生殖激素檢測試劑。

• 交叉反應研究: 研究 alpha 亞基在不同促性腺激素（如 LH, FSH, TSH）中的保守性。

4. 注意事項

• 分泌穩(wěn)定性: 雜交瘤細胞在多次傳代后可能丟失產生抗體的能力。建議定期通過 ELISA 檢測細胞上清液的抗體滴度。

• 抗體分型: 建議在使用前確認該克隆分泌抗體的同種型，以便于后續(xù)純化。

English Datasheet

1. General Product Information

• Product Name: BioVector? H80X Anti-hCG alpha Hybridoma Cell Line

• Antibody Type: Monoclonal Antibody (mAb)

• Specificity: Human Chorionic Gonadotropin alpha subunit (hCG alpha)

• Host Background: Balb/c Mouse

• Growth Properties: Semi-suspension

• Morphology: Typical hybridoma morphology; spherical cells with uniform size

2. Culture Conditions

• Basal Medium: BioVector? RPMI 1640

• Serum Supplement: 10% to 15% BioVector? Fetal Bovine Serum (FBS)

• Supplements: 2 mM L-Glutamine, 1 mM Sodium Pyruvate

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:5 ratio; maintain density between 200,000 and 1,000,000 cells/mL

3. Applications

• Antibody Production: Large-scale manufacturing of anti-hCG alpha mAbs via in vitro bioreactors or in vivo mouse ascites methods.

• Diagnostic Assay Development: Core component for pregnancy tests and reproductive hormone screening.

  

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• Structural Biology: Probing the common alpha-subunit epitopes shared by the glycoprotein hormone family.

4. Key Usage Notes

• Stability Monitoring: Hybridoma lines can drift or lose antibody secretion over time. BioVector? recommends frequent titer checks of the culture supernatant via ELISA.

• Isotype Verification: Confirm the immunoglobulin isotype to optimize purification strategies using Protein A or Protein G chromatography.

注意 / Note: H80X 雜交瘤細胞應在生物安全二級 (BSL-2) 條件下操作。在進行大規(guī)?？贵w生產前，建議先進行單細胞克隆化以確?？贵w分泌的一致性。H80X hybridoma cells should be handled under Biosafety Level 2 (BSL-2) conditions. Before large-scale antibody production, BioVector? suggests performing single-cell cloning to ensure the homogeneity and stability of antibody secretion.

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