BioVector? AY-27 大鼠膀胱癌細(xì)胞系 / BioVector? AY-27 Rat Bladder Cancer Cell Line

通用定義 / General Definition:

BioVector? AY-27 是一種廣泛使用的大鼠膀胱移行細(xì)胞癌 (Urothelial/Transitional Cell Carcinoma, TCC) 細(xì)胞系。該細(xì)胞系最初是通過(guò)向 Fischer 344 (F344) 大鼠膀胱內(nèi)灌注致癌物質(zhì)（如 $N$-丁基-$N$-(4-羥丁基)亞硝胺，BBN）誘導(dǎo)產(chǎn)生的。AY-27 的核心價(jià)值在于其能夠在大鼠體內(nèi)建立原位 (Orthotopic) 膀胱癌模型，高度模擬人類膀胱癌的生長(zhǎng)環(huán)境、組織學(xué)特征以及對(duì)藥物的反應(yīng)。它被公認(rèn)為研究膀胱癌免疫療法（如 BCG 灌注）、光動(dòng)力療法 (PDT) 以及局部化療藥物遞送的“金標(biāo)準(zhǔn)”大鼠模型。

BioVector? AY-27 is a widely utilized Rat Bladder Transitional Cell Carcinoma (TCC) cell line. It was originally derived from an $N$-butyl-$N$-(4-hydroxybutyl)nitrosamine (BBN)-induced bladder tumor in Fischer 344 (F344) rats. The primary significance of AY-27 lies in its ability to establish orthotopic bladder cancer models, which closely mimic the microenvironment, histological progression, and therapeutic responses of human bladder cancer. It is recognized as a premier model for investigating intravesical therapies, such as BCG immunotherapy, Photodynamic Therapy (PDT), and localized drug delivery systems.

BioVector? AY-27 技術(shù)說(shuō)明書(shū) (Technical Datasheet)

中文版說(shuō)明書(shū) (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? AY-27 大鼠膀胱癌細(xì)胞

• 組織來(lái)源： F344 大鼠膀胱移行上皮 (Urothelium)

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 細(xì)胞形態(tài)： 上皮樣，通常呈鋪路石狀排列。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI 1640。

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代比例： 1:3 至 1:6；建議在細(xì)胞匯合度達(dá)到 80%-90% 時(shí)進(jìn)行傳代。

3. 細(xì)胞應(yīng)用

• 原位腫瘤模型： 通過(guò)導(dǎo)尿管將 AY-27 細(xì)胞灌注至 F344 大鼠膀胱內(nèi)（通常需預(yù)先輕微損傷膀胱黏膜），建立原位癌模型。

• 免疫治療研究： 評(píng)估卡介苗 (BCG) 或細(xì)胞因子對(duì)膀胱腫瘤微環(huán)境的免疫調(diào)節(jié)作用。

• 藥物動(dòng)力學(xué)： 測(cè)試經(jīng)尿道灌注藥物（如絲裂霉素、多柔比星）在膀胱壁的滲透性與抗腫瘤效能。

4. 注意事項(xiàng)

• 同源性： 為確保成瘤率，體內(nèi)實(shí)驗(yàn)必須使用 F344 大鼠，以避免免疫排斥。

• 成瘤控制： 該細(xì)胞系在體內(nèi)生長(zhǎng)極快，原位接種后需密切通過(guò)超聲或血尿情況監(jiān)測(cè)腫瘤進(jìn)展，防止大鼠過(guò)早死亡。

English Datasheet

1. General Product Information

• Product Name: BioVector? AY-27 Rat Bladder Cancer Cell Line

• Source: Bladder Urothelium of Fischer 344 (F344) rats

• Growth Properties: Adherent

• Morphology: Epithelial-like; typical cobblestone pattern.

2. Culture Conditions

• Basal Medium: BioVector? RPMI 1640.

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS).

• Incubation: 37°C, 5% $CO_2$.

• Subculturing: 1:3 to 1:6 ratio; subculture at 80%-90% confluence.

3. Applications

• Orthotopic Tumor Modeling: Cells are instilled intravesically into F344 rats (often following a brief chemical or mechanical pre-conditioning of the bladder wall) to study localized cancer growth.

• Immunotherapy Research: A standard model for studying BCG-mediated immune responses and the recruitment of inflammatory cells to the tumor site.

• Photodynamic Therapy (PDT): Widely used to test photosensitizers and laser-based ablation techniques for superficial bladder tumors.

4. Key Usage Notes

• Syngeneic Consistency:Fischer 344 rats must be used for in vivo studies to ensure successful engraftment and prevent immunological rejection of the cells.

• Aggressive Growth: AY-27 exhibits rapid proliferation in vivo. Researchers should perform regular monitoring (e.g., small animal ultrasound) to track tumor volume and ensure animal welfare protocols are met.

注意 / Note: AY-27 細(xì)胞在多次傳代后可能會(huì)降低其在體內(nèi)的成瘤效率。BioVector? 建議使用低代數(shù)細(xì)胞進(jìn)行體內(nèi)實(shí)驗(yàn)，并驗(yàn)證細(xì)胞的形態(tài)一致性。

AY-27 cells may show a decline in orthotopic engraftment efficiency after extensive passaging. BioVector? recommends utilizing low-passage stocks for in vivo studies and periodically confirming epithelial morphology.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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