BioVector? PLum-AI 小鼠前列腺癌細(xì)胞系 / BioVector? PLum-AI Mouse Prostate Cancer Cell Line

通用定義 / General Definition:BioVector? PLum-AI 是一種源自轉(zhuǎn)基因小鼠模型的人工工程化前列腺癌細(xì)胞系。該細(xì)胞系通常建立在 PTEN 缺失和 P53 缺失的遺傳背景下，模擬了人類晚期前列腺癌最常見的基因改變。"AI" 通常代表 "Androgen Independent"（雄激素非依賴性） 或 "Androgen Insensitive"（雄激素不敏感），意味著該細(xì)胞系即使在低雄激素水平下也能持續(xù)生長(zhǎng)。這使其成為研究去勢(shì)抵抗性前列腺癌 (CRPC)、神經(jīng)內(nèi)分泌分化以及腫瘤干細(xì)胞特性的重要實(shí)驗(yàn)工具。

BioVector? PLum-AI is an engineered mouse prostate cancer cell line derived from transgenic mouse models. It is typically established on a genetic background of PTEN loss and P53 loss, mimicking the most frequent genomic alterations found in advanced human prostate cancer. The "AI" designation generally stands for "Androgen Independent" or "Androgen Insensitive", indicating the line's ability to proliferate despite low androgen levels. This makes it an essential experimental tool for studying Castration-Resistant Prostate Cancer (CRPC), neuroendocrine differentiation, and cancer stem cell characteristics.

BioVector? PLum-AI 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? PLum-AI 小鼠前列腺癌細(xì)胞

• 組織來源： 小鼠前列腺組織 (PTEN/P53 缺陷型)

• 分子特征：PTEN 缺失；P53 缺失；雄激素受體 (AR) 表達(dá)可能下調(diào)或發(fā)生功能轉(zhuǎn)變；表現(xiàn)為去勢(shì)抵抗特性。

• 生長(zhǎng)特性： 貼壁生長(zhǎng)。

• 細(xì)胞形態(tài)： 上皮樣或紡錘形，具有較強(qiáng)的異質(zhì)性，可見明顯的惡性轉(zhuǎn)化特征。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? PrEGM（前列腺上皮細(xì)胞生長(zhǎng)培養(yǎng)基）或 DMEM/F-12 (1:1)。

• 血清添加： 5% - 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)（在研究雄激素不敏感性時(shí)，常使用活性炭/糊精處理的胎牛血清 CT-FBS）。

• 補(bǔ)充因子： 胰島素、表皮生長(zhǎng)因子 (EGF) 以及牛垂體提取物 (BPE)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2。

• 傳代比例： 1:3 至 1:6。

3. 細(xì)胞應(yīng)用

• CRPC 機(jī)制研究： 探索前列腺癌如何從依賴雄激素狀態(tài)演變?yōu)榉且蕾嚑顟B(tài)。

• 神經(jīng)內(nèi)分泌轉(zhuǎn)分化： 研究 PTEN/P53 雙缺失如何誘導(dǎo)腫瘤向惡性程度更高的神經(jīng)內(nèi)分泌表型轉(zhuǎn)化。

• 同種異體移植模型： 由于其源自小鼠，可在具有免疫能力的同源小鼠（如 C57BL/6 背景）中接種，用于研究腫瘤免疫微環(huán)境。

4. 注意事項(xiàng)

• 血清敏感性： 雄激素水平對(duì)該細(xì)胞的表型維持至關(guān)重要。若要維持其“AI”特性，建議在無酚紅培養(yǎng)基中配合 CT-FBS 培養(yǎng)。

• 接種密度： 細(xì)胞在低密度下可能生長(zhǎng)較慢，建議保持適中的接種壓力。

English Datasheet

1. General Product Information

• Product Name: BioVector? PLum-AI Mouse Prostate Cancer Cell Line

• Source: Mouse prostate tissue (PTEN/P53 deficient model)

• Molecular Profile:PTEN deletion; P53 deletion; Androgen Receptor (AR) signaling may be bypassed or altered; exhibits castration-resistant characteristics.

• Growth Properties: Adherent.

• Morphology: Epithelial-like or spindle-shaped; exhibits high heterogeneity and distinct features of malignant transformation.

2. Culture Conditions

• Basal Medium: BioVector? PrEGM (Prostate Epithelial Cell Growth Medium) or DMEM/F-12 (1:1).

• Serum Supplement: 5% - 10% BioVector? Fetal Bovine Serum (FBS). For studying androgen independence, Charcoal-Stripped FBS (CT-FBS) is strictly required.

• Supplements: Insulin, Epidermal Growth Factor (EGF), and Bovine Pituitary Extract (BPE).

• Incubation: 37°C, 5% CO2.

• Subculturing: 1:3 to 1:6 ratio.

3. Applications

• CRPC Mechanistic Studies: Investigating the molecular switch from androgen-dependent to androgen-independent growth.

• Neuroendocrine Differentiation (NED): Studying how the dual loss of PTEN and P53 drives the transdifferentiation into aggressive neuroendocrine phenotypes.

• Syngeneic Models: Being a mouse line, it can be implanted into immunocompetent mice (e.g., C57BL/6 background) to study tumor-immune system interactions.

4. Key Usage Notes

• Hormone Control: To accurately maintain and study the androgen-insensitive phenotype, avoid using phenol red-containing media and use charcoal-stripped serum to eliminate exogenous steroids.

• Phenotypic Stability: Regular monitoring of lineage markers (such as E-cadherin or Synaptophysin) is recommended to track potential neuroendocrine transdifferentiation during long-term culture.

注意 / Note: BioVector? PLum-AI 應(yīng)在生物安全二級(jí) (BSL-2) 條件下操作。由于該細(xì)胞系涉及復(fù)雜的轉(zhuǎn)基因背景，建議在實(shí)驗(yàn)開始前通過 Western Blot 驗(yàn)證 PTEN 和 P53 的缺失狀態(tài)。BioVector? PLum-AI should be handled under Biosafety Level 2 (BSL-2) conditions. Given the complex transgenic background of the line, BioVector? recommends verifying the PTEN and P53 status via Western Blot prior to initiating key experiments.

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