BioVector? PLum-AD 小鼠前列腺癌細胞系 / BioVector? PLum-AD Mouse Prostate Cancer Cell Line

通用定義 / General Definition:

BioVector? PLum-AD 是一種源自轉(zhuǎn)基因小鼠模型的工程化前列腺癌細胞系。該細胞系與 PLum-AI 屬于同一家族，通常建立在 PTEN 缺失和 P53 缺失的遺傳背景下。"AD" 代表 "Androgen Dependent"（雄激素依賴型）。這意味著該細胞系的生長和存活高度依賴于雄激素信號通路。它是研究前列腺癌早期病變、雄激素受體（AR）信號轉(zhuǎn)導以及探索從激素敏感性向激素非依賴性演變機制的關(guān)鍵對照模型。

BioVector? PLum-AD is an engineered mouse prostate cancer cell line derived from transgenic mouse models. Typically established on a PTEN-null and P53-null genetic background, the "AD" designation stands for "Androgen Dependent". This indicates that the proliferation and survival of this cell line are strictly dependent on androgen signaling. It serves as a pivotal model for studying early-stage prostate carcinogenesis, androgen receptor (AR) signal transduction, and the mechanisms underlying the transition from hormone-sensitive to hormone-refractory disease.

BioVector? PLum-AD 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? PLum-AD 小鼠前列腺癌細胞

• 組織來源： 小鼠前列腺組織 (PTEN/P53 缺陷型模型)

• 分子特征：PTEN 缺失；P53 缺失；維持高水平的雄激素受體 (AR) 表達及功能活性。

• 生長特性： 貼壁生長。

• 細胞形態(tài)： 典型的小鼠前列腺上皮樣形態(tài)，排列較緊密。

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? PrEGM 或 DMEM/F-12 (1:1)。

• 血清添加： 5% - 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 激素補充： 培養(yǎng)基中通常需要添加合成雄激素（如 1-10 nM R1881）以維持其雄激素依賴性表型。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代比例： 1:3 至 1:5。

3. 細胞應用

• AR 信號研究： 研究雄激素與其受體結(jié)合后如何調(diào)控下游靶基因（如 PSA 同源物）的表達。

• 激素敏感性篩選： 評估新型抗雄激素藥物（如恩雜魯胺前體）的體外藥效。

• 演變模型構(gòu)建： 通過長期脫離雄激素培養(yǎng)，觀察其向 PLum-AI 表型轉(zhuǎn)化的過程。

4. 注意事項

• 雄激素維持： 若在不含雄激素的條件下（如使用 CT-FBS）培養(yǎng)，細胞生長會顯著減緩并可能進入凋亡，因此必須確保培養(yǎng)體系中雄激素的穩(wěn)定供應。

• 分化狀態(tài)： 隨著傳代次數(shù)增加，細胞可能出現(xiàn)自發(fā)性的激素脫敏，建議在實驗中使用低代數(shù)細胞。

English Datasheet

1. General Product Information

• Product Name: BioVector? PLum-AD Mouse Prostate Cancer Cell Line

• Source: Mouse prostate tissue (PTEN/P53 deficient model)

• Molecular Profile:PTEN deletion; P53 deletion; maintains robust Androgen Receptor (AR) expression and signaling activity.

• Growth Properties: Adherent.

• Morphology: Classic mouse prostatic epithelial morphology; cells tend to grow in organized clusters.

2. Culture Conditions

• Basal Medium: BioVector? PrEGM or DMEM/F-12 (1:1).

• Serum Supplement: 5% - 10% BioVector? Fetal Bovine Serum (FBS).

• Hormone Supplement: Requires supplementation with synthetic androgens (e.g., 1-10 nM R1881) to maintain the androgen-dependent growth phenotype.

• Incubation: 37°C, 5% $CO_2$.

• Subculturing: 1:3 to 1:5 ratio.

3. Applications

• AR Signaling Studies: Investigating the transcriptional regulation of downstream target genes following androgen binding.

• Hormone Sensitivity Assays: Evaluating the efficacy of novel anti-androgen compounds or AR degraders (PROTACs).

• Progression Modeling: Utilizing the line as a baseline to study the molecular triggers of castration resistance by inducing androgen deprivation in vitro.

4. Key Usage Notes

• Androgen Dependency: Proliferation is severely impaired in androgen-depleted media (e.g., media containing Charcoal-Stripped FBS without R1881). Consistent androgen levels are necessary for routine maintenance.

• Phenotypic Integrity: To avoid spontaneous drift toward an androgen-independent state, BioVector? recommends using low-passage cells and periodically verifying AR expression levels.

注意 / Note: BioVector? PLum-AD 應在生物安全二級 (BSL-2) 條件下操作。在進行涉及雄激素拮抗的實驗時，建議同時使用 PLum-AI 作為陰性對照，以增強實驗結(jié)論的準確性。

BioVector? PLum-AD should be handled under Biosafety Level 2 (BSL-2) conditions. When performing androgen antagonism assays, BioVector? recommends using the PLum-AI line as a negative control to ensure the specificity of the observed biological effects.

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