BioVector? CHLA-15 人神經(jīng)母細胞瘤細胞系 / BioVector? CHLA-15 Human Neuroblastoma Cell Line

通用定義 / General Definition:BioVector? CHLA-15 是一種源自人類神經(jīng)母細胞瘤 (Neuroblastoma) 的細胞系。該細胞系建立于一名接受過強力聯(lián)合化療后的復(fù)發(fā)患兒的腫瘤組織。CHLA-15 在神經(jīng)腫瘤研究中具有極高的臨床代表性，因為它保留了治療誘導(dǎo)的耐藥特征。該細胞系最顯著的分子特征包括 MYCN 基因擴增，這是神經(jīng)母細胞瘤預(yù)后不良的關(guān)鍵遺傳學(xué)標志。它常被用于研究腫瘤復(fù)發(fā)機制、耐藥性演變以及開發(fā)針對 MYCN 擴增型腫瘤的新型靶向藥物。

BioVector? CHLA-15 is a human cell line derived from Neuroblastoma. It was established from a tumor specimen obtained from a pediatric patient at the time of disease relapse following intensive multi-agent chemotherapy. CHLA-15 is highly clinically relevant as it retains features of therapy-induced resistance. A defining molecular hallmark of this line is the amplification of the MYCN oncogene, a critical prognostic factor for high-risk neuroblastoma. It is extensively utilized to investigate mechanisms of tumor recurrence, the evolution of drug resistance, and the development of novel targeted therapies for MYCN-amplified malignancies.

BioVector? CHLA-15 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? CHLA-15 人神經(jīng)母細胞瘤細胞

• 組織來源： 腹后膜腫瘤（復(fù)發(fā)性神經(jīng)母細胞瘤）

• 分子特征：MYCN 擴增；通常表現(xiàn)為對多種化療藥物（如烷化劑）的敏感性降低。

• 生長特性： 貼壁生長（細胞可能形成多層簇狀結(jié)構(gòu)）。

• 細胞形態(tài)： 上皮樣及小圓細胞，胞漿較少，常可見神經(jīng)突樣延伸。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? IMDM 或 DMEM/F-12 (1:1)。

• 血清添加： 10% - 20% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 補充因子： 建議添加 1x ITS (胰島素-轉(zhuǎn)移蛋白-硒) 以支持細胞在低密度時的活力。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2。

• 傳代比例： 1:2 至 1:4；細胞生長較緩慢且具有接觸抑制減弱的特點。

3. 細胞應(yīng)用

• 耐藥機制研究： 對比初治細胞系，研究復(fù)發(fā)性腫瘤對順鉑、長春新堿等藥物的耐藥分子途徑。

• MYCN 靶向研究： 利用該模型測試針對 MYCN 轉(zhuǎn)錄活性或相關(guān)代謝途徑的抑制劑。

• 小鼠異種移植模型： 建立 CDX (Cell Line-Derived Xenograft) 模型，用于評估藥物的體內(nèi)藥效。

4. 注意事項

• 細胞聚集： CHLA-15 具有較強的聚集傾向，傳代消化時需小心吹打以獲得單細胞懸液，否則會影響計數(shù)準確性和接種均勻度。

• 培養(yǎng)基變色： 復(fù)發(fā)性腫瘤細胞代謝活躍，產(chǎn)酸較多，需注意觀察 pH 值變化并定期（每周 2-3 次）更換培養(yǎng)基。

English Datasheet

1. General Product Information

• Product Name: BioVector? CHLA-15 Human Neuroblastoma Cell Line

• Source: Retroperitoneal tumor (Relapsed Neuroblastoma)

• Molecular Profile:MYCN Amplification; characterized by decreased sensitivity to standard chemotherapeutic agents such as alkylators.

• Growth Properties: Adherent (tends to form multi-layered clusters).

• Morphology: Epithelial-like and small round cells with scant cytoplasm; often displays short neurite-like processes.

2. Culture Conditions

• Basal Medium: BioVector? IMDM or DMEM/F-12 (1:1).

• Serum Supplement: 10% - 20% BioVector? Fetal Bovine Serum (FBS).

• Supplements: Supplementation with 1x ITS (Insulin-Transferrin-Selenium) is recommended to maintain viability at low seeding densities.

• Incubation: 37°C, 5% CO2.

• Subculturing: 1:2 to 1:4 ratio; growth is relatively slow compared to some primary neuroblastoma lines.

3. Applications

• Drug Resistance Profiling: Comparing relapsed cell phenotypes against treatment-naive models to identify molecular pathways of acquired resistance.

• Targeting MYCN: Utilizing the line as a model for testing inhibitors targeting MYCN transcription, protein stability, or downstream metabolic effectors.

• In Vivo Modeling: Creating CDX (Cell Line-Derived Xenograft) models to evaluate the pharmacodynamics of novel neuroblastoma drug candidates.

4. Key Usage Notes

• Cluster Management: CHLA-15 cells naturally grow in clusters. During trypsinization, gentle but thorough trituration is required to achieve a single-cell suspension for accurate experimental setup.

• Nutrient Demand: Due to the metabolic profile of relapsed tumor cells, regular media changes (2-3 times per week) are necessary to prevent the accumulation of lactic acid and depletion of glucose.

注意 / Note: BioVector? CHLA-15 應(yīng)在生物安全二級 (BSL-2) 條件下操作。鑒于該細胞系源自復(fù)發(fā)腫瘤，其表型可能在多次傳代后發(fā)生微小漂移，建議定期通過 FISH 驗證 MYCN 的擴增狀態(tài)。BioVector? CHLA-15 should be handled under Biosafety Level 2 (BSL-2) conditions. Given its origin from a relapsed tumor, the phenotype may undergo minor drift after extensive passaging; BioVector? recommends periodically verifying MYCN amplification status via FISH.

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