BioVector? Mycoplasma pneumoniae ATCC 29342 菌株 / BioVector? Mycoplasma pneumoniae ATCC 29342 Strain

通用定義 / General Definition:

BioVector? Mycoplasma pneumoniae ATCC 29342（又稱(chēng) M129 菌株）是肺炎支原體的國(guó)際標(biāo)準(zhǔn)參考菌株及全基因組測(cè)序模式株。作為柔膜菌綱 (Mollicutes) 的一員，它天然缺乏細(xì)胞壁。該菌株是人類(lèi)原發(fā)性非典型肺炎（又稱(chēng)“行走性肺炎”）的主要致病原。它通過(guò)其特殊的頂端結(jié)構(gòu)（主要依賴(lài) P1 黏附蛋白）附著在宿主呼吸道上皮細(xì)胞上，引起纖毛停滯和細(xì)胞損傷。在代謝途徑上，與依賴(lài)精氨酸的人型支原體不同，ATCC 29342 主要通過(guò)發(fā)酵葡萄糖來(lái)獲取能量，產(chǎn)生乳酸和乙酸。

BioVector? Mycoplasma pneumoniae ATCC 29342 (also known as strain M129) is the internationally recognized reference strain and the whole-genome sequenced type model for Mycoplasma pneumoniae. Belonging to the class Mollicutes, it is characterized by its natural lack of a rigid cell wall. This strain is a primary causative agent of primary atypical pneumonia (often referred to as "walking pneumonia") in humans. It attaches to host respiratory epithelium via a specialized terminal organelle (heavily reliant on the P1 adhesin protein), causing ciliostasis and cellular damage. Metabolically, unlike the arginine-hydrolyzing Mycoplasma hominis, ATCC 29342 derives energy primarily through the fermentation of glucose, yielding lactic and acetic acids.

BioVector? ATCC 29342 技術(shù)說(shuō)明書(shū) (Technical Datasheet)

中文版說(shuō)明書(shū) (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱(chēng)： BioVector? 肺炎支原體 (Mycoplasma pneumoniae) ATCC 29342

• 其他編號(hào)： M129 菌株 (Subtype 1)

• 形態(tài)特征： 具有多形性（球狀、細(xì)絲狀或帶有特殊的“瓶頸”狀頂端附著細(xì)胞器），由于無(wú)細(xì)胞壁，革蘭氏染色無(wú)效。

• 主要毒力因子： P1 黏附素 (P1 Adhesin)、社區(qū)獲得性呼吸道窘迫綜合征 (CARDS) 毒素、過(guò)氧化氫分泌。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? SP4 葡萄糖肉湯或瓊脂（如 ATCC Medium 2611），必須富含血清和酵母提取物。

• 代謝指示： 培養(yǎng)基中含葡萄糖和酚紅。由于其發(fā)酵葡萄糖產(chǎn)酸，培養(yǎng)基的 pH 值會(huì)下降，顏色會(huì)由紅色/橙色變?yōu)辄S色。

• 大氣環(huán)境： 液體培養(yǎng)可好氧進(jìn)行；固體平板必須在 5% $CO_2$ 環(huán)境下培養(yǎng)。

• 培養(yǎng)溫度： 37 攝氏度。

3. 菌株應(yīng)用

• 疫苗與藥物研發(fā)： 作為評(píng)估大環(huán)內(nèi)酯類(lèi)（如阿奇霉素）、氟喹諾酮類(lèi)和四環(huán)素類(lèi)抗生素體外抑菌效果 (MIC) 的金標(biāo)準(zhǔn)菌株。

• 致病機(jī)制研究： 研究 P1 黏附蛋白介導(dǎo)的細(xì)胞粘附機(jī)制及 CARDS 毒素對(duì)氣道上皮細(xì)胞的致病性。

• 分子診斷開(kāi)發(fā)： 提取高質(zhì)量的基因組 DNA，用于呼吸道多重 PCR 檢測(cè)試劑盒的研發(fā)、驗(yàn)證與質(zhì)控。

4. 注意事項(xiàng)

• 生長(zhǎng)極度緩慢： 肺炎支原體生長(zhǎng)非常緩慢，液體培養(yǎng)通常需要 1 到 3 周才開(kāi)始顯現(xiàn)指示劑變色。液體通常不會(huì)出現(xiàn)明顯的渾濁。

• 傳代時(shí)機(jī)： 當(dāng)液體培養(yǎng)基顏色從紅色變?yōu)?strong data-path-to-node="13,1,0" data-index-in-node="19" style="line-height:1.15 !important;margin-top:0px !important;">橙色或淺黃色時(shí)為最佳傳代或凍存時(shí)機(jī)。一旦完全變黃，培養(yǎng)基過(guò)酸會(huì)導(dǎo)致細(xì)胞迅速死亡。

English Datasheet

1. General Product Information

• Product Name: BioVector? Mycoplasma pneumoniae ATCC 29342

• Alternative Designations: Strain M129 (Subtype 1)

• Morphology: Highly pleomorphic (spherical, filamentous, or flask-shaped with a specialized terminal attachment organelle). Unresponsive to Gram staining.

• Key Virulence Factors: P1 Adhesin, Community-Acquired Respiratory Distress Syndrome (CARDS) toxin, and secretion of hydrogen peroxide ($H_2O_2$).

2. Culture Conditions

• Basal Media: BioVector? SP4 Glucose Broth or Agar (e.g., ATCC Medium 2611), heavily supplemented with animal serum and yeast extract.

• Growth Indicator: Contains glucose and phenol red. Fermentation of glucose produces acid, lowering the pH and causing a color shift from red/orange to yellow.

• Environment: Liquid cultures can be grown aerobically; agar plates require a 5% $CO_2$ atmosphere to prevent drying and maintain optimal pH.

• Incubation Temperature: 37°C.

3. Applications

• Antimicrobial Research: Serves as the gold-standard reference strain for testing the in vitro susceptibility (MIC assays) of macrolides, fluoroquinolones, and tetracyclines.

• Pathogenesis Studies: Investigating host-cell interactions, specifically the structural biology of the attachment organelle and the cellular damage induced by CARDS toxin.

• Assay Standardization: Providing molecular-grade nucleic acids to validate and calibrate multiplex respiratory PCR panels.

4. Key Usage Notes

• Prolonged Incubation:M. pneumoniae is exceptionally slow-growing. Broth cultures may take 7 to 21 days to show metabolic activity. Expected growth presents as a color change, not macroscopic turbidity.

• Harvest Timing: The optimal window for subculturing or cryopreservation is when the medium turns orange to light yellow. If the medium becomes bright yellow, the low pH will rapidly induce cell death.

注意 / Note: 在固體瓊脂上，ATCC 29342 形成的菌落極其微?。ㄍǔ?< 0.1 mm），往往不像其他支原體那樣呈現(xiàn)典型的“油煎蛋”形態(tài)，而是表現(xiàn)為致密的球形或顆粒狀。必須使用立體顯微鏡 (10x - 40x) 才能進(jìn)行形態(tài)學(xué)觀察和菌落計(jì)數(shù)。

On solid agar, colonies of ATCC 29342 are microscopic (typically < 0.1 mm) and often lack the classic "fried-egg" appearance seen in other Mycoplasma species, instead appearing as dense, granular spheres. A stereomicroscope (10x - 40x magnification) is strictly required for morphological observation and colony counting.

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