BioVector? 解脲支原體 ATCC 33175 菌株 / BioVector? Ureaplasma urealyticum ATCC 33175 Strain

重要澄清 / Important Clarification: > 之前的 ATCC 33175 通常指代口腔病原菌 Aggregatibacter actinomycetemcomitans。如果您所指的是 解脲支原體 (Ureaplasma urealyticum)，其標(biāo)準(zhǔn)的模式菌株通常為 ATCC 27618 (T960)。然而，針對(duì)解脲支原體系列科研需求，我們?yōu)槟峁┤缦箩槍?duì) Ureaplasma urealyticum 的通用技術(shù)說明。

通用定義 / General Definition:

BioVector? 解脲支原體是一種極小的、缺乏細(xì)胞壁的柔膜菌綱 (Mollicutes) 微生物。它是人類泌尿生殖道中常見的共生菌，但在特定條件下可引發(fā)非淋菌性尿道炎、不孕不育、絨毛膜羊膜炎及新生兒呼吸道疾病。其代謝上最顯著的特征是具有高度活躍的尿素酶 (Urease)，通過水解尿素產(chǎn)生氨來獲得能量（產(chǎn)生質(zhì)子梯度）。由于缺乏細(xì)胞壁，它對(duì)內(nèi)酰胺類抗生素天然耐藥。

BioVector? Ureaplasma urealyticum is an extremely small, cell-wall-deficient microorganism belonging to the class Mollicutes. It is a common commensal of the human urogenital tract but can become pathogenic, causing non-gonococcal urethritis, infertility, chorioamnionitis, and neonatal respiratory infections. Its most defining metabolic trait is the presence of highly active urease, which hydrolyzes urea to generate ammonia and create a proton motive force for energy. Being cell-wall-less, it is naturally resistant to beta-lactam antibiotics.

BioVector? 解脲支原體技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? 解脲支原體 (Ureaplasma urealyticum)

• 基因組特征： 基因組極?。s 0.75-1.0 Mbp），高度依賴宿主或培養(yǎng)基提供營養(yǎng)。

• 形態(tài)特征： 多形性（球形、梨形），直徑僅 0.1-1.0 $\mu$m；革蘭氏染色不顯色，需用 Giemsa 染色。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? Ureaplasma 培養(yǎng)基 (10號(hào)培養(yǎng)基)，需含有牛血清、尿素和生長因子。

• pH 要求： 初始 pH 需精確調(diào)至 6.0 ($\pm 0.2$)（該菌在酸性環(huán)境下生長較好）。

• 大氣環(huán)境： 需在 5% $CO_2$ 環(huán)境下培養(yǎng)。

• 代謝指示： 培養(yǎng)基中含酚紅，當(dāng)尿素被水解產(chǎn)生氨導(dǎo)致 pH 升高時(shí)，培養(yǎng)基顏色會(huì)由黃變紅。

3. 菌株應(yīng)用

• 抗生素耐藥性監(jiān)測： 研究大環(huán)內(nèi)酯類、四環(huán)素類及喹諾酮類藥物對(duì)生殖道支原體的療效。

• 生殖健康研究： 探討解脲支原體對(duì)精子活力、粘附及胚胎發(fā)育的影響。

• 分子診斷對(duì)照： 作為 PCR 或恒溫?cái)U(kuò)增檢測盒的陽性對(duì)照標(biāo)準(zhǔn)品。

4. 注意事項(xiàng)

• 細(xì)胞死亡風(fēng)險(xiǎn)： 由于該菌產(chǎn)氨會(huì)導(dǎo)致培養(yǎng)基 pH 迅速升高，一旦變紅必須立即轉(zhuǎn)種或冷凍保存，否則細(xì)菌會(huì)因堿中毒迅速死亡。

• 過濾除菌： 由于其體積極小，可通過 0.45 $\mu$m 濾膜，實(shí)驗(yàn)室操作需防范污染。

English Datasheet

1. General Product Information

• Product Name: BioVector? Ureaplasma urealyticum

• Genomic Profile: Highly reduced genome (~0.75-1.0 Mbp), showing extreme dependency on external nutrient sources.

• Morphology: Pleomorphic (spherical to pear-shaped), 0.1-1.0 $\mu$m in diameter; lacks a peptidoglycan cell wall and does not respond to Gram staining.

2. Culture Conditions

• Medium: BioVector? Ureaplasma Medium (Medium 10), enriched with horse/bovine serum, urea, and growth supplements.

• pH Optimization: Initial pH must be set to 6.0 ($\pm 0.2$).

• Environment: 37°C in a 5% $CO_2$ incubator.

• Growth Indicator: Phenol red in the medium shifts from yellow to pink/red as urease activity produces ammonia, raising the pH.

3. Applications

• Antimicrobial Susceptibility Testing (AST): Monitoring resistance trends to macrolides, tetracyclines, and fluoroquinolones.

• Reproductive Medicine: Investigating the impact of Ureaplasma colonization on sperm motility and maternal-fetal health.

• Diagnostic Standardization: Serving as a quantitative positive control for molecular assays (e.g., qPCR, LAMP).

4. Key Usage Notes

• Toxicity Warning: The metabolic byproduct (ammonia) is toxic to the bacteria. Cultures must be harvested or subcultured immediately upon color change to prevent "overgrowth" death.

• Biosafety: Classified as Biosafety Level 2 (BSL-2). Requires stringent aseptic technique and disposal protocols for urogenital pathogens.

注意 / Note: 解脲支原體在固體培養(yǎng)基上形成的菌落極小，通常需要使用低倍顯微鏡才能觀察到典型的“油煎蛋”或顆粒狀形態(tài)。

Colonies of Ureaplasma urealyticum on agar are microscopic; observation typically requires a low-power microscope to visualize the characteristic "fried-egg" or granular appearance.

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