BioVector? ACH-3P 人絨毛膜癌細胞系 / BioVector? ACH-3P Human Choriocarcinoma Cell Line

通用定義 / General Definition:

BioVector? ACH-3P 是一種源自人絨毛膜癌 (Choriocarcinoma) 的細胞系。它是由 ACH-3L（原始人絨毛膜癌細胞系）通過在小鼠體內(nèi)進行連續(xù)傳代和體外克隆篩選而建立的。與母本細胞相比，ACH-3P 表現(xiàn)出更強的侵襲力、更快的生長速度以及穩(wěn)定的激素分泌能力（如分泌 hCG - 人絨毛膜促性腺激素）。它是研究滋養(yǎng)層細胞生物學(xué)、胎盤發(fā)育異常、腫瘤侵襲轉(zhuǎn)移機制以及評估胎盤屏障藥物轉(zhuǎn)運的重要模型。

BioVector? ACH-3P is a human cell line derived from Choriocarcinoma. It was established from ACH-3L (the original line) through serial passage in mice followed by in vitro clonal selection. Compared to its parental line, ACH-3P exhibits enhanced invasiveness, higher proliferation rates, and stable hormone secretion profiles, particularly the secretion of hCG (human Chorionic Gonadotropin). It serves as an essential model for studying trophoblast biology, placental development disorders, mechanisms of tumor invasion and metastasis, and evaluating drug transport across the placental barrier.

BioVector? ACH-3P 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? ACH-3P 人絨毛膜癌細胞

• 組織來源： 胎盤/絨毛膜 (Placenta/Choriocarcinoma)

• 生長特性： 貼壁生長

• 細胞形態(tài)： 上皮樣，常呈簇狀生長，可見典型的滋養(yǎng)層巨細胞特征。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? Ham's F-12 培養(yǎng)基 或 DMEM/F-12 (1:1)。

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代比例： 1:3 至 1:6；建議在細胞匯合度達到 80%-90% 時進行傳代。

3. 細胞應(yīng)用

• 生殖生物學(xué)： 研究合體滋養(yǎng)層細胞的分化過程及激素合成（hCG、孕酮）。

• 侵襲與轉(zhuǎn)移： 利用其高侵襲特性，研究基質(zhì)金屬蛋白酶 (MMPs) 在腫瘤擴散中的作用。

• 胎盤毒理學(xué)： 構(gòu)建體外胎盤屏障模型，評估藥物或環(huán)境毒素對胚胎發(fā)育的潛在影響。

4. 注意事項

• 分泌物監(jiān)測： 該細胞系持續(xù)分泌 hCG，長期培養(yǎng)可能導(dǎo)致培養(yǎng)基 pH 值變化較快，需定期監(jiān)測并及時換液。

• 貼壁牢固度： ACH-3P 貼壁較牢固，消化時建議使用含 EDTA 的 0.25% 胰酶，并密切觀察細胞變圓情況以防止過度消化。

English Datasheet

1. General Product Information

• Product Name: BioVector? ACH-3P Human Choriocarcinoma Cell Line

• Tissue Source: Placenta/Choriocarcinoma

• Growth Properties: Adherent

• Morphology: Epithelial-like; cells often grow in clusters and exhibit characteristic features of trophoblastic giant cells.

2. Culture Conditions

• Basal Medium: BioVector? Ham's F-12 Medium or DMEM/F-12 (1:1).

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS).

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:3 to 1:6 ratio; subculture when cells reach 80%–90% confluence.

3. Applications

• Reproductive Biology: Investigating syncytiotrophoblast differentiation and hormone biosynthesis (hCG, progesterone).

• Invasion and Metastasis: Leveraging its high-invasion phenotype to study the role of Matrix Metalloproteinases (MMPs) in malignancy.

• Placental Toxicology: Establishing in vitro placental barrier models to assess the impact of drugs or environmental toxins on fetal development.

4. Key Usage Notes

• Hormone Secretion: ACH-3P continuously secretes hCG into the medium, which can influence local metabolic environments; BioVector? recommends frequent medium changes for optimal health.

• Dissociation: Due to strong adherence, 0.25% Trypsin-EDTA is recommended for subculturing. Carefully monitor for cell rounding to avoid damaging membrane receptors via over-digestion.

注意 / Note: BioVector? ACH-3P 應(yīng)在生物安全二級 (BSL-2) 條件下操作。該細胞系具有較高的異質(zhì)性，建議在關(guān)鍵實驗前通過免疫熒光驗證其滋養(yǎng)層標志物（如 Cytokeratin-7）的表達。

BioVector? ACH-3P should be handled under Biosafety Level 2 (BSL-2) conditions. Given the heterogeneity of choriocarcinoma lines, BioVector? recommends verifying the expression of trophoblast markers such as Cytokeratin-7 via immunofluorescence before critical experiments.

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

電話:400-800-2947

工作QQ/微信同號:1843439339

網(wǎng)址

http://www.nedfriskphoto.com