BioVector? Muribaculum intestinale 菌株 / BioVector? Muribaculum intestinale Strain

通用定義 / General Definition:BioVector? Muribaculum intestinale 是一種屬于擬桿菌目 (Bacteroidales)、Muribaculaceae 科（原稱 S24-7 家族）的革蘭氏陰性、專性厭氧、不產(chǎn)芽孢桿菌。該菌株是小鼠腸道微生物群中最占優(yōu)勢的成員之一，但在人類腸道中分布較少。M. intestinale 以其極強(qiáng)的復(fù)雜碳水化合物（如植物源性多糖）降解能力而聞名。作為 Muribaculaceae 科的模式物種，它是研究嚙齒動(dòng)物腸道微生態(tài)穩(wěn)態(tài)、宿主-微生物共進(jìn)化以及膳食纖維代謝對粘膜免疫影響的關(guān)鍵生物模型。

BioVector? Muribaculum intestinale is a Gram-negative, obligately anaerobic, non-spore-forming rod-shaped bacterium belonging to the order Bacteroidales and the family Muribaculaceae (formerly known as the S24-7 family). This strain is one of the most dominant members of the mouse gut microbiota, though it is less prevalent in humans.M. intestinale is renowned for its specialized capacity to degrade complex carbohydrates, particularly plant-derived polysaccharides. As the type species of its family, it serves as a pivotal biological model for studying rodent gut homeostasis, host-microbe co-evolution, and the impact of dietary fiber metabolism on mucosal immunity.

BioVector? M. intestinale 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? Muribaculum intestinale 菌株

• 其他編號： YL27, DSM 28989

• 基因組特征： 基因組中含有大量編碼碳水化合物活性酶（CAZymes）的基因，專門用于降解復(fù)雜的植物多糖和宿主粘蛋白。

• 主要代謝產(chǎn)物： 產(chǎn)生乙酸 (Acetate)、琥珀酸 (Succinate) 和丙酸 (Propionate)。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? 改良 PYG 培養(yǎng)基（含葡萄糖、酵母膏、蛋白胨）或 BHI 培養(yǎng)基，通常需添加血紅素和維生素 K1。

• 培養(yǎng)溫度： 37 攝氏度。

• 厭氧要求： 必須在嚴(yán)格厭氧環(huán)境下培養(yǎng)。

• 生長特性： 生長相對緩慢，在固體培養(yǎng)基上形成微小、半透明的菌落。

3. 菌株應(yīng)用

• 營養(yǎng)代謝研究： 研究不同種類的膳食纖維如何選擇性地富集 Muribaculaceae 科細(xì)菌。

• 腸道定殖實(shí)驗(yàn)： 在無菌 (Germ-free) 小鼠模型中重建腸道菌群，研究其對屏障功能的影響。

• 比較基因組學(xué)： 探討小鼠優(yōu)勢菌群與人類優(yōu)勢菌群（如擬桿菌屬）在功能位點(diǎn)上的異同。

4. 注意事項(xiàng)

• 培養(yǎng)挑戰(zhàn)： 該菌對培養(yǎng)條件較為苛刻，建議在培養(yǎng)基中添加少量的原代腸道內(nèi)容物提取物或特定的多糖底物以促進(jìn)初始生長。

• 保存建議： 建議使用含有 15-20% 甘油的厭氧凍存管于 -80°C 保存。

English Datasheet

1. General Product Information

• Product Name: BioVector? Muribaculum intestinale Strain

• Alternative Designations: YL27, DSM 28989

• Genomic Features: The genome is highly enriched with genes encoding Carbohydrate-Active Enzymes (CAZymes), specialized for the breakdown of complex plant polysaccharides and host-derived mucins.

• Metabolic Output: Produces acetate, succinate, and propionate as major fermentation end-products.

2. Culture Conditions

• Basal Medium: BioVector? Modified PYG (Peptone Yeast Glucose) or BHI broth, supplemented with hemin and vitamin K1.

• Incubation Temperature: 37°C.

• Anaerobic Requirement: Must be maintained under strictly anaerobic conditions.

• Growth Characteristics: Exhibits slow growth kinetics; forms minute, translucent colonies on anaerobic agar plates.

3. Applications

• Nutritional Metabolism: Investigating how specific dietary fibers selectively promote the expansion of Muribaculaceae populations.

• Gnotobiotic Research: Colonizing germ-free mouse models to study the impact of dominant commensals on mucosal immune maturation.

• Evolutionary Biology: Studying host-specific microbial adaptations and the niche partitioning between Muribaculaceae in rodents and Bacteroides in humans.

4. Key Usage Notes

• Cultivation Difficulty:M. intestinale can be fastidious; BioVector? suggests supplementing media with specific complex glycans to improve recovery from cryopreservation.

• Sample Integrity: Due to its dominance in mice, ensure that experimental cross-contamination from environmental mouse sources is strictly controlled when using this strain in defined consortia.

注意 / Note:Muribaculum intestinale 是了解小鼠模型中“健康”腸道狀態(tài)的基礎(chǔ)。BioVector? 提醒研究者，該菌在抗生素處理后極易受到抑制，但在高纖維飲食下會(huì)迅速恢復(fù)。Muribaculum intestinale is fundamental to understanding the "healthy" gut state in mouse models. BioVector? notes that while this strain is highly susceptible to antibiotic suppression, it recovers rapidly under high-fiber dietary regimens.

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