BioVector? AMPA-HEK293  AMPA受體穩(wěn)定表達細胞系 / BioVector? AMPA Receptor Stable Expression Cell Line

通用定義 / General Definition:

BioVector? AMPA 表達細胞系（通常構建于 HEK293 或 CHO 細胞）是穩(wěn)定轉(zhuǎn)染了人源或大鼠源 AMPA 型谷氨酸受體（如 GluA1, GluA2, GluA3 或 GluA4 亞基）的工程化細胞。AMPA 受體是中樞神經(jīng)系統(tǒng)中介導快速興奮性突觸傳遞的主要離子型受體。該細胞系通常還共表達輔助亞基（如 TARP $\gamma$-2 / Stargazin），以確保受體在細胞膜表面的正常定位和功能動力學。它是神經(jīng)科學研究、電生理分析以及篩選抗癲癇藥物和認知改善藥物的核心工具。

BioVector? AMPA Expression Cell Lines (typically hosted in HEK293 or CHO cells) are engineered lines stably transfected with human or rat AMPA-type glutamate receptor subunits (e.g., GluA1, GluA2, GluA3, or GluA4). AMPA receptors are the primary ionotropic receptors mediating fast excitatory synaptic transmission in the central nervous system. These lines often co-express auxiliary subunits (such as TARP $\gamma$-2 / Stargazin) to ensure proper membrane trafficking and functional kinetics. They serve as essential tools for neuroscience research, electrophysiological profiling, and screening for anti-epileptic or pro-cognitive therapeutic candidates.

BioVector? AMPA 受體表達細胞系技術說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? AMPA 受體 (GluA1-4) 穩(wěn)定表達細胞系

• 母本細胞： HEK293 或 CHO-K1

• 表達靶點： 谷氨酸離子型受體 AMPA 型（可提供同源或異源四聚體組合）

• 生長特性： 貼壁生長

• 篩選抗生素： 含有 G418、潮霉素 (Hygromycin) 或 嘌呤霉素 (Puromycin)，用于維持受體復合體的穩(wěn)定性。

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? DMEM 或 F12 培養(yǎng)基。

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 關鍵注意事項： 培養(yǎng)基中可能需要添加谷氨酸受體拮抗劑（如 CNQX），以防止受體過度激活產(chǎn)生的興奮性細胞毒性導致細胞死亡。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

3. 細胞應用

• 高通量篩選 (HTS)： 利用熒光成像板讀者 (FLIPR) 檢測鈣離子流或膜電位變化，篩選變構調(diào)節(jié)劑。

• 電生理研究： 利用全細胞膜片鉗 (Patch Clamp) 研究受體的激活、脫敏及恢復動力學。

• 突觸可塑性模擬： 研究受體亞基組成（如 GluA2 缺失導致的鈣滲透性變化）對神經(jīng)元功能的潛在影響。

4. 注意事項

• 受體脫敏： AMPA 受體脫敏極快（毫秒級）。在實驗中通常需要添加脫敏抑制劑（如 Cyclothiazide / CTZ）來獲得穩(wěn)定的電流記錄。

• 表達驗證： 建議定期進行免疫熒光染色，確認受體在細胞表面的分布。

English Datasheet

1. General Product Information

• Product Name: BioVector? AMPA Receptor (GluA1-4) Stable Expression Cell Line

• Parental Line: HEK293 or CHO-K1

• Target Target: Glutamate Ionotropic Receptor AMPA Type (Available as homo- or hetero-tetrameric configurations)

• Growth Properties: Adherent

• Selection Markers: Maintained with G418, Hygromycin, or Puromycin to ensure stable maintenance of the receptor and auxiliary subunit plasmids.

2. Culture Conditions

• Basal Medium: BioVector? DMEM or F12 Medium.

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS).

• Key Requirement: Media may require supplementation with a non-competitive antagonist (e.g., CNQX) to prevent excitotoxicity-induced cell death caused by spontaneous receptor activation.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

3. Applications

• High-Throughput Screening (HTS): Utilizing FLIPR assays for calcium flux or membrane potential changes to identify positive or negative allosteric modulators (PAMs/NAMs).

• Electrophysiology: Performing patch clamp recordings to characterize activation, deactivation, and desensitization kinetics.

• Drug Discovery: Evaluating novel compounds for the treatment of epilepsy, depression, or neurodegenerative diseases.

4. Key Usage Notes

• Desensitization Management: AMPA receptors exhibit extremely rapid desensitization. To obtain robust steady-state currents, the use of desensitization blockers like Cyclothiazide (CTZ) is often necessary during recordings.

• Passage Stability: Functional expression levels may decrease over time. BioVector? recommends verifying current density via patch clamp or ion imaging every 10 passages.

注意 / Note: BioVector? AMPA 受體表達細胞系應在生物安全二級 (BSL-2) 條件下操作。為獲得最佳實驗結(jié)果，請確保在電生理實驗中使用快速灌流系統(tǒng)，以捕捉受體的瞬時峰值電流。

BioVector? AMPA expression cell lines should be handled under Biosafety Level 2 (BSL-2) conditions. For optimal results, BioVector? recommends using a fast-application perfusion system in electrophysiology to accurately capture transient peak currents.

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