BioVector? iPTi001-A 人誘導(dǎo)多能干細(xì)胞系 / BioVector? iPTi001-A Human Induced Pluripotent Stem Cell Line

通用定義 / General Definition:

BioVector? iPTi001-A 是一種標(biāo)準(zhǔn)化的人源誘導(dǎo)多能干細(xì)胞 (iPSC) 系，通常被記錄為該系列中的首個(gè)參考株。該細(xì)胞系由健康供體或特定表型供體的體細(xì)胞（如外周血單核細(xì)胞或皮膚成纖維細(xì)胞）通過先進(jìn)的非整合技術(shù)重編程而成。iPTi001-A 嚴(yán)格遵循多能性標(biāo)準(zhǔn)，具有卓越的自我更新能力和全向分化潛能。它是基礎(chǔ)生物醫(yī)學(xué)研究、干細(xì)胞庫(kù)建設(shè)以及建立標(biāo)準(zhǔn)化分化方案（如向神經(jīng)或心肌譜系分化）的基石模型。

BioVector? iPTi001-A is a standardized human Induced Pluripotent Stem Cell (iPSC) line, often designated as the primary reference strain within its series. This line is reprogrammed from somatic cells (e.g., PBMCs or skin fibroblasts) of a healthy or phenotypically characterized donor using advanced non-integrative technologies. iPTi001-A strictly adheres to pluripotency standards, exhibiting exceptional self-renewal capacity and omnipotent differentiation potential. It serves as a foundational model for basic biomedical research, stem cell banking, and the establishment of standardized differentiation protocols, such as neural or cardiomyocyte lineage specification.

BioVector? iPTi001-A 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? iPTi001-A 人 iPSC 細(xì)胞系

• 重編程平臺(tái)： 非整合型（如塞內(nèi)卡病毒或游離質(zhì)粒），確保宿主基因組的原始性。

• 特性驗(yàn)證： * 標(biāo)志物： 強(qiáng)表達(dá) SSEA-4, TRA-1-60, TRA-1-81, OCT4 和 SOX2。

  • 三胚層能力： 已驗(yàn)證其在體內(nèi)（畸胎瘤形成）或體外（胚狀體 EB）形成三胚層的能力。

• 核型： 經(jīng)鑒定為正常人類二倍體（46, XX 或 46, XY）。

2. 培養(yǎng)條件

• 維持培養(yǎng)基： BioVector? mTeSR Plus 或同級(jí)別無蛋白、無喂養(yǎng)層體系。

• 表面包被：Matrigel 或 Laminin-511。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代建議： 采用 0.5 mM EDTA 進(jìn)行集群傳代（Clump passaging），每 4-6 天傳代一次，比例為 1:6 至 1:12。

3. 細(xì)胞應(yīng)用

• 發(fā)育模型： 研究人體從多能性狀態(tài)向特定器官前體細(xì)胞轉(zhuǎn)化的分子開關(guān)。

• 對(duì)照基準(zhǔn)： 作為各種疾病特異性 iPSC 實(shí)驗(yàn)中的野生型（WT）對(duì)照株。

• 再生醫(yī)學(xué)： 用于優(yōu)化細(xì)胞大規(guī)模擴(kuò)增工藝及臨床前安全評(píng)估。

4. 注意事項(xiàng)

• 環(huán)境穩(wěn)定性： iPSC 對(duì) pH 值和滲透壓變化極度敏感，建議每日固定時(shí)間換液。

• 存活增強(qiáng)： 在復(fù)蘇或進(jìn)行單細(xì)胞操作（如基因編輯后的克隆篩選）時(shí)，必須添加 10 $\mu$M ROCK 抑制劑 (Y-27632)。

English Datasheet

1. General Product Information

• Product Name: BioVector? iPTi001-A Human iPSC Line

• Reprogramming Platform: Non-integrative (e.g., Sendai virus or episomal vectors), ensuring no genomic scarring or integration.

• Characterization: * Markers: Robust expression of SSEA-4, TRA-1-60, TRA-1-81, OCT4, and SOX2.

  • Potency: Validated tri-lineage differentiation potential via in vivo (teratoma) or in vitro (embryoid body) assays.

• Karyotype: Confirmed normal human diploid karyotype.

2. Culture Conditions

• Maintenance Medium: BioVector? mTeSR Plus or equivalent feeder-free, chemically defined media.

• Coating Matrix:Matrigel or recombinant Laminin-511.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: Clump passaging using 0.5 mM EDTA is recommended; typical split ratios are 1:6 to 1:12 every 4–6 days.

3. Applications

• Developmental Modeling: Investigating molecular switches governing the transition from pluripotency to lineage-specific progenitor cells.

• Baseline Control: Utilized as a wild-type (WT) reference line in experiments involving disease-specific iPSC models.

• Regenerative Medicine: Optimizing large-scale cell expansion bioprocesses and performing pre-clinical safety assessments.

4. Key Usage Notes

• Environmental Consistency: iPSCs are highly sensitive to fluctuations in pH and osmolarity; BioVector? recommends media changes at a consistent time each day.

• Survival Enhancement: The addition of 10 $\mu$M ROCK inhibitor (Y-27632) is essential during thawing or single-cell procedures (e.g., clonal selection after gene editing) to prevent apoptosis.

注意 / Note: BioVector? iPTi001-A 應(yīng)在生物安全二級(jí) (BSL-2) 條件下操作。該細(xì)胞系經(jīng)過多代驗(yàn)證，表現(xiàn)出極佳的遺傳穩(wěn)定性和分化效率。

BioVector? iPTi001-A should be handled under Biosafety Level 2 (BSL-2) conditions. This line has been validated across multiple passages, demonstrating superior genetic stability and differentiation efficiency.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

電話:400-800-2947

工作QQ/微信同號(hào):1843439339

網(wǎng)址

http://www.nedfriskphoto.com