BioVector? ZZUi0024-A 人誘導(dǎo)多能干細胞系 / BioVector? ZZUi0024-A Human Induced Pluripotent Stem Cell Line

通用定義 / General Definition:

BioVector? ZZUi0024-A 是一種人源誘導(dǎo)多能干細胞 (iPSC) 系，屬于 ZZUi 標(biāo)準(zhǔn)化系列。該細胞系通常由特定臨床特征供體（或作為對照的健康供體）的體細胞通過非整合型重編程技術(shù)建立。ZZUi0024-A 具備典型的干細胞生物學(xué)特性，包括分化為內(nèi)、中、外三個胚層的能力以及在維持培養(yǎng)基中無限擴增的潛力。它是研究人類遺傳學(xué)、發(fā)育生物學(xué)以及進行細胞替代療法研究的重要工具。

BioVector? ZZUi0024-A is a human Induced Pluripotent Stem Cell (iPSC) line from the standardized ZZUi series. It is established from donor-derived somatic cells using non-integrative reprogramming technologies. ZZUi0024-A possesses classic biological hallmarks of stem cells, including the potential to differentiate into lineages of all three germ layers and the capacity for infinite expansion in maintenance media. It serves as a vital tool for human genetics, developmental biology, and cell-based regenerative medicine research.

BioVector? ZZUi0024-A 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? ZZUi0024-A 人 iPSC 細胞系

• 重編程載體： 非整合型（如塞內(nèi)卡病毒或 Episomal 質(zhì)粒），不改變宿主基因組結(jié)構(gòu)。

• 多能性鑒定： * 免疫熒光： OCT4, SOX2, NANOG, SSEA-4 陽性。

  • 堿性磷酸酶 (AP)： 強陽性染色。

• 核型穩(wěn)定性： 經(jīng) $G$-帶掃描驗證為正常二倍體。

2. 培養(yǎng)條件

• 維持體系： BioVector? mTeSR Plus 或 Essential 8 (E8) 培養(yǎng)基。

• 基質(zhì)要求： 需包被 Matrigel 或 Vitronectin (玻連蛋白)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代比例： 1:6 至 1:10，建議采用 EDTA 或 ReLeSR 進行小集群傳代。

3. 細胞應(yīng)用

• 器官類器官構(gòu)建： 誘導(dǎo)形成腦、腎或腸道類器官。

• 精準(zhǔn)醫(yī)療： 結(jié)合基因編輯技術(shù)研究特定位點突變的致病性。

• 分化篩選： 研究小分子化合物對多能性維持或定向分化的影響。

4. 注意事項

• 自發(fā)分化： 定期檢查集落。若出現(xiàn)非典型形態(tài)（如拉長、扁平細胞），應(yīng)及時手動剔除。

• ROCK 抑制劑： 復(fù)蘇及單細胞接種時，必須添加 10 $\mu$M Y-27632 以防止細胞凋亡。

English Datasheet

1. General Product Information

• Product Name: BioVector? ZZUi0024-A Human iPSC Line

• Reprogramming Vector: Non-integrative (e.g., Sendai virus or Episomal vectors), ensuring host genomic integrity.

• Pluripotency Validation: * Immunofluorescence: Positive for OCT4, SOX2, NANOG, and SSEA-4 markers.

  • Alkaline Phosphatase (AP): Strong positive staining.

• Karyotypic Stability: Confirmed normal diploid status via $G$-banding.

2. Culture Conditions

• Maintenance System: BioVector? mTeSR Plus or Essential 8 (E8) media.

• Coating Requirements: Plates must be pre-coated with Matrigel or Vitronectin.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:6 to 1:10 ratio; non-enzymatic small clump passaging via EDTA or ReLeSR is recommended.

3. Applications

• Organoid Generation: Inducing the formation of brain, kidney, or intestinal 3D organoids.

• Precision Medicine: Combining with CRISPR/Cas9 to investigate the pathogenicity of specific genetic variants.

• Compound Screening: Studying the effects of small molecules on pluripotency maintenance or lineage-specific differentiation.

4. Key Usage Notes

• Monitoring Differentiation: Inspect colonies daily. Any atypical morphologies (e.g., elongated, fibroblastic cells) should be manually removed to preserve culture purity.

• Cell Survival: BioVector? recommends adding 10 $\mu$M ROCK inhibitor (Y-27632) during thawing and single-cell seeding to mitigate dissociation-induced apoptosis.

注意 / Note: BioVector? ZZUi0024-A 應(yīng)在生物安全二級 (BSL-2) 條件下操作。該細胞系是研究人體細胞生物學(xué)和建立穩(wěn)定分化協(xié)議的高效模型。

BioVector? ZZUi0024-A should be handled under Biosafety Level 2 (BSL-2) conditions. This cell line provides a robust model for human cell biology research and the establishment of stable differentiation protocols.

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

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