BioVector? ZZUi0013-A 人誘導(dǎo)多能干細胞系 / BioVector? ZZUi0013-A Human Induced Pluripotent Stem Cell Line

通用定義 / General Definition:

BioVector? ZZUi0013-A 是一種標準化的人源誘導(dǎo)多能干細胞 (iPSC) 系。該細胞系通常是由特定臨床背景的供體（或健康對照）的體細胞，通過先進的非整合重編程技術(shù)（如塞內(nèi)卡病毒或游離質(zhì)粒）重編程而成。ZZUi 系列通常與特定的醫(yī)學(xué)研究中心或大學(xué)（如鄭州大學(xué)，Zhengzhou University）相關(guān)聯(lián)。ZZUi0013-A 具有典型的多能性特征，能夠無限增殖并分化為所有三個胚層的細胞。它為研究人類疾病的發(fā)病機制、藥物篩選及發(fā)育生物學(xué)提供了高質(zhì)量的細胞模型。

BioVector? ZZUi0013-A is a standardized human Induced Pluripotent Stem Cell (iPSC) line. This line is established from donor-derived somatic cells using advanced non-integrative reprogramming technologies (such as Sendai virus or episomal vectors). The ZZUi series is typically associated with specific clinical cohorts or academic institutions (e.g., Zhengzhou University). ZZUi0013-A exhibits classic pluripotency characteristics, including the capacity for infinite self-renewal and the potential to differentiate into cells of all three germ layers. It serves as a high-quality cellular model for investigating human disease mechanisms, drug screening, and developmental biology.

BioVector? ZZUi0013-A 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? ZZUi0013-A 人 iPSC 細胞系

• 供體 background： 詳細的供體臨床背景（如特定疾病表型或健康對照）。

• 重編程方法： 非整合型（Non-integrative），確?；蚪M中不含有外源載體序列。

• 特性驗證： * 標志物： 強表達 OCT4, NANOG, SOX2, SSEA4。

  • 核型： 具有正常的二倍體核型。

• 生長特性： 貼壁生長，集落邊緣清晰。

2. 培養(yǎng)條件

• 維持培養(yǎng)基： BioVector? mTeSR Plus 或同級別無喂養(yǎng)層培養(yǎng)基。

• 基質(zhì)包被：Matrigel 或 Laminin-511。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代比例： 1:5 至 1:8，每 4-5 天傳代一次。

3. 細胞應(yīng)用

• 疾病建模： 若該系源自特定疾病患者，可用于模擬該疾病的細胞病理過程。

• 分化研究： 分化為功能性神經(jīng)元、心肌細胞或類器官（Organoids）。

• 安全性篩選： 評估候選藥物在人源背景下的潛在毒性。

4. 注意事項

• 細胞密度： 避免細胞過度匯合（Over-confluence），否則會增加自發(fā)分化的風(fēng)險。

• 復(fù)蘇建議： 復(fù)蘇后前 24 小時建議使用含 ROCK 抑制劑 (Y-27632) 的培養(yǎng)基以提高存活率。

English Datasheet

1. General Product Information

• Product Name: BioVector? ZZUi0013-A Human iPSC Line

• Donor Profile: Detailed clinical background (e.g., specific disease phenotype or healthy control).

• Reprogramming Method: Non-integrative, ensuring the host genome remains free of vector integration.

• Characterization:

  • Markers: Strong expression of OCT4, NANOG, SOX2, and SSEA4.

  • Karyotype: Validated normal diploid karyotype.

• Growth Properties: Adherent; forms well-defined, compact colonies.

2. Culture Conditions

• Maintenance Medium: BioVector? mTeSR Plus or equivalent feeder-free system.

• Coating Substrate:Matrigel or Laminin-511.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:5 to 1:8 ratio; typically passaged every 4–5 days.

3. Applications

• Disease Modeling: If derived from a specific patient, it serves to model the cytopathological processes of that disease.

• Lineage Differentiation: Differentiation into functional neurons, cardiomyocytes, or 3D organoids.

• Safety Pharmacology: Evaluating the toxicological profile of drug candidates in a human-specific genetic context.

4. Key Usage Notes

• Differentiation Control: Monitor the culture daily for spontaneous differentiation. Differentiated areas should be removed prior to passaging.

• Thawing Protocol: BioVector? recommends including a ROCK inhibitor (Y-27632) in the medium for the first 24 hours post-thaw to significantly improve cell attachment and viability.

注意 / Note: BioVector? ZZUi0013-A 應(yīng)在生物安全二級 (BSL-2) 條件下操作。該細胞系對營養(yǎng)供應(yīng)較為敏感，建議嚴格按照 BioVector? 推薦的換液頻率進行維護。

BioVector? ZZUi0013-A should be handled under Biosafety Level 2 (BSL-2) conditions. The line is sensitive to nutrient fluctuations; BioVector? recommends strict adherence to the feeding schedule to maintain optimal pluripotency.

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