BioVector? SPPHli004-A 人誘導(dǎo)多能干細(xì)胞系 / BioVector? SPPHli004-A Human Induced Pluripotent Stem Cell Line

通用定義 / General Definition:

BioVector? SPPHli004-A 是一種人源誘導(dǎo)多能干細(xì)胞 (iPSC) 系，它是 SPPHli 系列標(biāo)準(zhǔn)化細(xì)胞株中的重要成員。該細(xì)胞系由健康供體的體細(xì)胞通過(guò)非整合重編程技術(shù)（通常為塞內(nèi)卡病毒或游離質(zhì)粒）建立。SPPHli004-A 具有典型的人多能干細(xì)胞特征，能夠無(wú)限自我更新，并在特定誘導(dǎo)條件下分化為體內(nèi)任何類(lèi)型的細(xì)胞。該細(xì)胞系經(jīng)過(guò)嚴(yán)格的質(zhì)量控制，包括核型分析、多能性標(biāo)志物檢測(cè)及三胚層分化能力驗(yàn)證，常作為生物醫(yī)學(xué)研究中的標(biāo)準(zhǔn)對(duì)照株或分化研究的基礎(chǔ)模型。

BioVector? SPPHli004-A is a human Induced Pluripotent Stem Cell (iPSC) line, a key member of the standardized SPPHli series. This line was established from somatic cells of a healthy donor using non-integrative reprogramming technologies (typically Sendai virus or episomal vectors). SPPHli004-A exhibits classic human pluripotent stem cell characteristics, capable of infinite self-renewal and differentiation into any cell type of the human body under specific inductive conditions. The line undergoes rigorous quality control, including karyotyping, pluripotency marker testing, and tri-lineage differentiation validation, serving as a standard control line or a foundational model for differentiation studies in biomedical research.

BioVector? SPPHli004-A 技術(shù)說(shuō)明書(shū) (Technical Datasheet)

中文版說(shuō)明書(shū) (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱(chēng)： BioVector? SPPHli004-A 人 iPSC 細(xì)胞系

• 供體背景： 健康供體，具有明確的性別及年齡背景資料。

• 重編程方式： 非整合型（Non-integrative），無(wú)病毒基因組殘留。

• 質(zhì)量鑒定： 已通過(guò) $G$-帶核型掃描（46, XX 或 46, XY）以及無(wú)支原體檢測(cè)。

• 生長(zhǎng)特性： 貼壁生長(zhǎng)，在優(yōu)質(zhì)基質(zhì)上形成邊緣清晰、細(xì)胞致密的集落。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)體系： BioVector? mTeSR Plus 或同類(lèi)無(wú)蛋白/無(wú)喂養(yǎng)層培養(yǎng)基。

• 包被材料：Matrigel (基質(zhì)膠) 或重組人 Laminin-511。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代策略： 推薦使用 0.5 mM EDTA 進(jìn)行離解，采用小集群傳代（Small Clumps），傳代比例通常為 1:6 至 1:10。

3. 細(xì)胞應(yīng)用

• 遺傳學(xué)研究： 作為對(duì)照組，評(píng)估疾病相關(guān)突變對(duì)細(xì)胞功能的影響。

• 細(xì)胞治療探索： 研究多能干細(xì)胞向臨床相關(guān)細(xì)胞類(lèi)型（如心肌、神經(jīng)、造血）的分化效率。

• 高通量篩選： 用于藥物發(fā)現(xiàn)過(guò)程中早期的人源毒性測(cè)試和藥效評(píng)估。

4. 注意事項(xiàng)

• 分化風(fēng)險(xiǎn)： 若集落中心出現(xiàn)空洞或邊緣出現(xiàn)分化的成纖維樣細(xì)胞，需立即進(jìn)行手工清理（Scraping）。

• 儲(chǔ)存： 長(zhǎng)期儲(chǔ)存于液氮?dú)庀啵?span id="06asuiaw0cey" class="math-inline" data-math="-150" data-index-in-node="14" style="line-height:1.15 !important;margin-top:0px !important;">$-150$ 攝氏度以下）。

English Datasheet

1. General Product Information

• Product Name: BioVector? SPPHli004-A Human iPSC Line

• Donor Profile: Derived from a healthy donor; comprehensive demographic data available.

• Reprogramming Method: Non-integrative technology, ensuring the host genome remains free of vector sequences.

• Quality Control: Validated via $G$-banded karyotyping (46, XX or 46, XY) and mycoplasma-free certification.

• Growth Properties: Adherent; forms dense colonies with distinct borders on high-quality substrates.

2. Culture Conditions

• Culture System: BioVector? mTeSR Plus or equivalent protein-free/feeder-free media.

• Coating Substrate:Matrigel or recombinant human Laminin-511.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: Recommended dissociation using 0.5 mM EDTA for small clump passaging; typical split ratios range from 1:6 to 1:10.

3. Applications

• Genetic Research: Serving as a baseline control to evaluate the impact of disease-related mutations on cellular phenotypes.

• Cell Therapy Development: Investigating the differentiation efficiency into clinically relevant lineages (e.g., cardiomyocytes, neurons, hematopoietic cells).

• High-Throughput Screening: Utilized for early-stage human-derived toxicity testing and efficacy evaluation in drug discovery.

4. Key Usage Notes

• Differentiation Management: Monitor daily. If hollow centers or fibroblastic-like cells appear at colony edges, perform manual scraping to maintain culture purity.

• Cryopreservation: Store in the vapor phase of liquid nitrogen (below $-150$ degrees Celsius) for long-term viability.

注意 / Note: BioVector? SPPHli004-A 應(yīng)在生物安全二級(jí) (BSL-2) 條件下操作。該細(xì)胞系是構(gòu)建等基因?qū)φ?(Isogenic Controls) 的理想母本。在進(jìn)行基因組編輯時(shí)，建議配合 BioVector? 專(zhuān)用轉(zhuǎn)染試劑盒以提高成功率。

BioVector? SPPHli004-A should be handled under Biosafety Level 2 (BSL-2) conditions. This line is an ideal parental candidate for generating isogenic controls. For genomic editing, BioVector? recommends using specialized transfection kits to optimize efficiency.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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