BioVector? SPPHli003-A 人誘導(dǎo)多能干細(xì)胞系 / BioVector? SPPHli003-A Human Induced Pluripotent Stem Cell Line

通用定義 / General Definition:

BioVector? SPPHli003-A 是一種標(biāo)準(zhǔn)化的人源誘導(dǎo)多能干細(xì)胞 (iPSC) 系。該細(xì)胞系通常由健康供體的體細(xì)胞（如外周血單核細(xì)胞 PBMC 或皮膚成纖維細(xì)胞）通過非整合型重編程技術(shù)（如塞內(nèi)卡病毒或游離質(zhì)粒）制備而成。SPPHli003-A 被廣泛用作正常對照株 (Control Line)，用于與攜帶特定疾病突變的 iPSC 進(jìn)行比較研究。它具有自我更新能力和分化為內(nèi)、中、外三個胚層所有細(xì)胞類型的潛能。

BioVector? SPPHli003-A is a standardized human Induced Pluripotent Stem Cell (iPSC) line. This line is typically reprogrammed from somatic cells (such as PBMCs or skin fibroblasts) of a healthy donor using non-integrative technologies (e.g., Sendai virus or episomal vectors). SPPHli003-A is widely utilized as a normal control line for comparative studies with disease-specific iPSC models. It possesses the capacity for infinite self-renewal and the potential to differentiate into all cell types of the three germ layers (ectoderm, mesoderm, and endoderm).

BioVector? SPPHli003-A 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? SPPHli003-A 人 iPSC 細(xì)胞系

• 供體背景： 健康供體，通常包含完整的臨床背景及人口統(tǒng)計學(xué)資料。

• 重編程方式： 非整合型技術(shù)，確保宿主基因組無外源基因殘留。

• 多能性標(biāo)志物： 高表達(dá) OCT4, NANOG, SOX2, SSEA4, TRA-1-60 及 TRA-1-81。

• 生長特性： 貼壁生長，呈致密的集落狀（Colony）。

2. 培養(yǎng)條件

• 維持培養(yǎng)基： BioVector? mTeSR Plus 或 mTeSR1 無喂養(yǎng)層培養(yǎng)體系。

• 包被基質(zhì)： 必須使用 基質(zhì)膠 (Matrigel) 或 Laminin-511 進(jìn)行預(yù)包被。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代方式： 建議使用 EDTA 或 ReLeSR 進(jìn)行非酶類成簇傳代，以保持細(xì)胞干性。

3. 細(xì)胞應(yīng)用

• 對照研究： 作為基因編輯實驗（如 CRISPR/Cas9）的母本細(xì)胞或疾病模型的野生型對照。

• 發(fā)育生物學(xué)： 研究人類早期胚胎發(fā)育過程。

• 藥物毒性評估： 分化為心肌細(xì)胞、肝細(xì)胞或神經(jīng)元，用于評估藥物的早期安全性。

4. 注意事項

• 自發(fā)分化： 需每日觀察。若集落邊緣出現(xiàn)扁平、散亂的細(xì)胞，說明發(fā)生自發(fā)分化，需及時清除。

• 核型監(jiān)測： 建議每 10-15 代進(jìn)行一次 G 帶核型分析，以確保染色體結(jié)構(gòu)正常。

English Datasheet

1. General Product Information

• Product Name: BioVector? SPPHli003-A Human iPSC Line

• Donor Profile: Derived from a healthy donor; comprehensive clinical and demographic data are typically available.

• Reprogramming Method: Non-integrative methods to ensure no exogenous DNA integration into the host genome.

• Pluripotency Markers: Strong expression of OCT4, NANOG, SOX2, SSEA4, TRA-1-60, and TRA-1-81.

• Growth Properties: Adherent; grows in dense, well-defined colonies.

2. Culture Conditions

• Maintenance Medium: BioVector? mTeSR Plus or mTeSR1 (Feeder-free system).

• Surface Coating: Plates must be pre-coated with Matrigel or Laminin-511.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: Non-enzymatic clump passaging using EDTA or ReLeSR is highly recommended to maintain pluripotency.

3. Applications

• Control Studies: Serving as the parental line for gene editing (e.g., CRISPR/Cas9) or as a wild-type control for disease-specific iPSC models.

• Developmental Biology: Modeling early human embryonic development and lineage specification.

• Drug Toxicology: Differentiation into specialized cells (cardiomyocytes, hepatocytes, or neurons) for early-stage drug safety screening.

4. Key Usage Notes

• Spontaneous Differentiation: Monitor daily. Differentiated cells (characterized by flattened or enlarged morphology at colony edges) should be mechanically removed or avoided during passaging.

• Genomic Integrity: BioVector? recommends routine G-banded karyotyping every 10–15 passages to verify chromosomal stability.

注意 / Note: BioVector? SPPHli003-A 應(yīng)在生物安全二級 (BSL-2) 條件下操作。該細(xì)胞系對操作者的傳代技術(shù)要求較高，建議在實驗前進(jìn)行標(biāo)準(zhǔn)化培訓(xùn)以確保集落質(zhì)量。

BioVector? SPPHli003-A should be handled under Biosafety Level 2 (BSL-2) conditions. This cell line requires high proficiency in passaging techniques; BioVector? suggests standardized training to ensure the maintenance of high-quality pluripotent colonies.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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