BioVector? Thy0517 人胸腺瘤細(xì)胞系 / BioVector? Thy0517 Human Thymoma Cell Line

通用定義 / General Definition:

BioVector? Thy0517 是一種源自人胸腺瘤 (Thymoma) 組織的特異性細(xì)胞系。胸腺瘤是源于胸腺上皮細(xì)胞的腫瘤，臨床上常與重癥肌無力（Myasthenia Gravis）等自身免疫性疾病密切相關(guān)。Thy0517 細(xì)胞系保留了胸腺上皮細(xì)胞的關(guān)鍵生化特征，包括特定細(xì)胞角蛋白的表達(dá)以及參與 T 細(xì)胞選擇的微環(huán)境模擬能力。它是研究胸腺腫瘤生物學(xué)、胸腺上皮與 T 細(xì)胞相互作用以及副腫瘤綜合征發(fā)病機(jī)制的重要體外模型。

BioVector? Thy0517 is a specialized cell line derived from human Thymoma tissue. Thymomas are tumors originating from the thymic epithelial cells and are frequently associated with paraneoplastic autoimmune conditions, most notably Myasthenia Gravis (MG). The Thy0517 cell line retains essential biochemical characteristics of thymic epithelia, including the expression of specific cytokeratins and the ability to simulate the microenvironment involved in T-cell selection. It serves as a vital in vitro model for studying thymic oncology, epithelial-T cell interactions, and the pathogenesis of thymus-related autoimmune syndromes.

BioVector? Thy0517 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? Thy0517 人胸腺瘤細(xì)胞

• 組織來源： 胸腺 (Thymus)

• 疾病背景： 胸腺瘤 (Thymoma)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮樣，通常呈多角形，具有典型的上皮細(xì)胞超微結(jié)構(gòu)。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM/F12 混合培養(yǎng)基。

• 血清添加： 10% - 15% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 關(guān)鍵添加劑： 某些實(shí)驗(yàn)方案建議添加上皮細(xì)胞生長因子 (EGF) 和氫化可的松以促進(jìn)生長。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代比例： 1:2 至 1:4；建議在細(xì)胞匯合度達(dá)到 80-90% 時(shí)傳代。

3. 細(xì)胞應(yīng)用

• 腫瘤生物學(xué)： 研究胸腺瘤細(xì)胞的增殖信號(hào)通路（如 EGFR、IGF-1R 通路）。

• 免疫學(xué)模擬： 與原代 T 細(xì)胞共培養(yǎng)，研究胸腺上皮腫瘤對(duì) T 細(xì)胞發(fā)育和自身耐受的影響。

• 藥物篩選： 測(cè)試針對(duì)難治性或轉(zhuǎn)移性胸腺癌的新型化療藥物和靶向療法。

4. 注意事項(xiàng)

• 異質(zhì)性： 胸腺瘤細(xì)胞在體外培養(yǎng)中可能表現(xiàn)出一定的形態(tài)異質(zhì)性，需定期觀察。

• 驗(yàn)證： 建議通過檢測(cè)細(xì)胞角蛋白（Cytokeratins）或抗原呈遞分子（MHC II）來確認(rèn)其上皮屬性。

English Datasheet

1. General Product Information

• Product Name: BioVector? Thy0517 Human Thymoma Cell Line

• Tissue Source: Thymus

• Disease Context: Thymoma

• Growth Properties: Adherent

• Morphology: Epithelial-like; typically polygonal with characteristic epithelial ultrastructures.

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM/F12 (1:1) mixture.

• Serum Supplement: 10% - 15% BioVector? Fetal Bovine Serum (FBS).

• Supplements: Recombinant human EGF and Hydrocortisone may be added to optimize the growth of the epithelial lineage.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:2 to 1:4 ratio; subculture when the monolayer reaches 80-90% confluence.

3. Applications

• Cancer Research: Investigating oncogenic signaling pathways (e.g., EGFR, IGF-1R) specific to thymic epithelial tumors.

• Immunological Modeling: Co-culture studies with T-lymphocytes to examine how thymomas disrupt central tolerance and T-cell education.

• Drug Discovery: Screening therapeutic compounds for the treatment of advanced thymic malignancies and associated autoimmune disorders.

4. Key Usage Notes

• Phenotypic Stability: Epithelial markers may fluctuate with high passage numbers; BioVector? recommends verifying the expression of cytokeratins to ensure cell identity.

• Adhesion Profile: Thy0517 cells require a stable surface for attachment; ensure the use of high-quality tissue culture-treated flasks.

注意 / Note: BioVector? Thy0517 應(yīng)在生物安全二級(jí) (BSL-2) 條件下操作。由于其來源于人體腫瘤組織，所有實(shí)驗(yàn)廢棄物必須嚴(yán)格按照生物危險(xiǎn)品處理規(guī)范進(jìn)行處置。

BioVector? Thy0517 should be handled under Biosafety Level 2 (BSL-2) conditions. Given its human tumor origin, all experimental waste must be disposed of in strict accordance with biohazardous material protocols.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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